Cervical cancer (CC) is the most familiar gynecological malignancy. With the poor prognosis of CC patients, this study explored the effect of microRNA (miR)‐130b‐5p targeting ELK1 expression on self‐renewal ability and stemness of CC stem cells. The tissues of patients with CC or cervical benign lesions were collected. MiR‐130b‐5p and ELK1 expression was detected by reverse transcription quantitative polymerase chain reaction and western blot analysis. Human CC cell line Hela was cultured and the induced CC stem cells were introduced with miR‐130b‐5p mimic or silenced ELK1 to figure their roles in self‐renewal ability, stemness, colony formation, proliferation, migration, invasion abilities, and apoptosis of CC stem cells. Tumor growth was detected in nude mice in vivo. The targeting relationship between miR‐130b‐5p and ELK1 was analyzed using bioinformatic prediction and dual luciferase reporter gene assay. Decreased miR‐130b‐5p and elevated ELK1 existed in CC tissues of patients. Up‐regulated miR‐130b‐5p decreased ELK1 expression in CC stem cells. Elevated miR‐130b‐5p or silenced ELK1 inhibited self‐renewal ability and stemness, colony formation, proliferation, migration and invasion abilities, promoted apoptosis of CC stem cells, as well as decreased the weight and volume of tumor in nude mice. ELK1 was found to be targeted by miR‐130b‐5p. Overexpression ELK1 effectively reversed the cellular phenotypic changes and tumor formation in vivo caused by up‐regulation of miR‐130b‐5p. We conclude that up‐regulated miR‐130b‐5p or silenced ELK1 inhibits CC stem cell growth.

中文翻译：

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升高的 microRNA ‐130b-5p 或沉默的 ELK1 抑制自我更新能力、增殖、迁移和侵袭能力，并促进宫颈癌干细胞凋亡

宫颈癌（CC）是最常见的妇科恶性肿瘤。由于CC患者预后不良，本研究探讨了靶向ELK1表达的microRNA (miR)-130b-5p对CC干细胞自我更新能力和干细胞的影响。收集CC或宫颈良性病变患者的组织。通过逆转录定量聚合酶链反应和蛋白质印迹分析检测 MiR-130b-5p 和 ELK1 的表达。培养人 CC 细胞系 Hela，将诱导的 CC 干细胞引入 miR-130b-5p 模拟物或沉默的 ELK1，以确定它们在自我更新能力、干性、集落形成、增殖、迁移、侵袭能力和凋亡中的作用。 CC干细胞。在体内的裸鼠中检测到肿瘤生长。使用生物信息学预测和双荧光素酶报告基因分析分析 miR-130b-5p 和 ELK1 之间的靶向关系。患者的 CC 组织中存在 miR-130b-5p 降低和 ELK1 升高。上调的 miR-130b-5p 降低了 CC 干细胞中 ELK1 的表达。升高的 miR-130b-5p 或沉默的 ELK1 抑制了裸鼠的自我更新能力和干性、集落形成、增殖、迁移和侵袭能力，促进了 CC 干细胞的凋亡，并减少了肿瘤的重量和体积。发现 ELK1 被 miR-130b-5p 靶向。过表达 ELK1 有效地逆转了由 miR-130b-5p 上调引起的体内细胞表型变化和肿瘤形成。我们得出结论，上调的 miR-130b-5p 或沉默的 ELK1 会抑制 CC 干细胞的生长。