Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening.

需要大量高质量的 iPSC 系来克服由遗传背景效应引起的供体相关变异。我们使用仙台病毒重编程从两名健康女性的真皮成纤维细胞中产生了两个人类 iPSC 系。 iPSC 系的质量评估证实了多能性标记的表达、三系分化能力以及重编程因子的外源表达的缺失。两种 iPSC 系均具有与供体成纤维细胞系相匹配的基因型，遗传稳定。这些 iPSC 系添加到可用的参考系中，作为多基因和多因素疾病的疾病建模资源，用于评估分化方案和毒理学筛查。