Salt stress is a serious abiotic stressor impeding plant growth and crop production around the world. Plant glycosyltransferases are thought to serve important roles in dealing with stress conditions, however, the functional role of how UGTs cope with salt stress is not well understood. Carex rigescens (Franch.) V. Krecz, is a widely distributed species of turfgrass with strong salinity tolerance found in northern China. To investigate how the glycosyltransferase gene, CrUGT87A1, functions in C. rigescens, we performed analyses of cloning, transcriptional expression, subcellular localization, and overexpression. The full-length sequence of CrUGT87A1 is 1455 bp with a 1338 bp length ORF, which encodes 445 amino acids, while CrUGT87A1 was found to be a nuclear and plasmalemma-localized protein. We found that the transcriptional expression of CrUGT87A1 was up-regulated under ABA, heat, salt, and drought treatments in leaf tissues. CrUGT87A1 overexpression in Arabidopsis plants had a significantly higher germination rate, better growth and physiology, and a higher expression levels of transcripts related to salt stress-related genes under high-salinity conditions, suggesting that CrUGT87A1 is involved in salt tolerance. The transcriptional expression of genes related to flavonoid-synthesis related and the flavonoid content reflected higher accumulations of flavonoids in transgenic plants. Our study demonstrated that CrUGT87A1 could play an important role in resisting salt stress due to increased flavonoid accumulation, which can promote antioxidation when dealing with high-salinity conditions. This study advances our collective understanding of the functional role of UGTs and can be used to improve the salt tolerance and breeding of crops and plants.

盐胁迫是严重的非生物胁迫源，阻碍了世界各地的植物生长和作物生产。植物糖基转移酶被认为在应对胁迫条件中起着重要作用，但是，UGTs如何应对盐胁迫的功能性作用尚不清楚。北方草（Carex rigescens）（弗兰克（Franch。））研究如何糖基转移酶基因，CrUGT87A1，在功能C. rigescens，我们进行了克隆，转录表达，亚细胞定位，和过表达的分析。CrUGT87A1的全长序列它是1455 bp，长度为1338 bp的ORF，编码445个氨基酸，而CrUGT87A1是一种位于核和浆膜定位的蛋白质。我们发现CrUGT87A1的转录表达在ABA，热，盐和干旱处理下在叶片组织中被上调。在高盐度条件下，拟南芥植物中的CrUGT87A1过表达具有显着更高的发芽率，更好的生长和生理学以及与盐胁迫相关基因相关的转录本的表达水平，这表明CrUGT87A1参与耐盐性。类黄酮合成相关基因的转录表达及类黄酮含量反映了转基因植物中类黄酮的较高积累。我们的研究表明，由于类黄酮积累增加，CrUGT87A1可以在抵抗盐胁迫中起重要作用，当处理高盐度条件时，它可以促进抗氧化作用。这项研究提高了我们对UGT的功能作用的集体理解，可用于提高耐盐性和农作物和植物的育种。