In this study, we report a novel indigenous Bacillus thuringiensis (Bt) isolate, T26, which showed spores and crystals under scanning electron microscope and pathogenicity against the pink bollworm (Pectinophora gossypiella Saunders) in artificial diet based bioassay. SDS-PAGE analysis of the spore-crystal mixture of the Bt isolate, T26 revealed presence of three major protein bands of approximate molecular weights of 80, 55 and 40 kDa. The draft genome assembly consists of 56 scaffolds with an entire draft genome size of 5,054,095 bp. NCBI blast analysis revealed that assembled draft genome is spread over in a chromosome (4,818,543 bp) and one plasmid (235,552 bp). NCBI Prokaryotic Genome Annotation Pipeline (PGAP) showed presence of 5033 coding gene sequences and 159 RNAs genes. None of the known lepidopteran active genes (cry1, cry2 and cry9) could be detected with PCR or with whole genome sequence analysis using Bt toxin scanner tool or CryProcessor tool. Thus, presence of protein crystals and toxicity towards cotton pink bollworm and absence of any known cry/vip/cyt type of genes in draft genome indicates it is a novel type of Bt isolate. Further investigation of this genome sequence along with protein sequencing will lead to understand the novel factors responsible for its virulence and could be a useful tool for the insect resistance management in pink bollworm.

在这项研究中，我们报告了一种新型的本土苏云金芽孢杆菌（Bt）苏云金芽孢杆菌T26，在扫描电子显微镜下显示出孢子和晶体，并在基于人工饮食的生物测定中显示了对粉红色棉铃虫（Pectinophora gossypiella Saunders）的致病性。Bt孢子晶体混合物的SDS-PAGE分析在分离物中，T26揭示了三个主要蛋白带的存在，其分子量分别约为80、55和40 kDa。基因组草图集由56个支架组成，整个基因组草图大小为5,054,095 bp。NCBI blast分析显示，组装的草图基因组分布在一条染色体（4,818,543 bp）和一个质粒（235,552 bp）中。NCBI原核基因组注释管道（PGAP）显示存在5033个编码基因序列和159个RNA基因。PCR或使用Bt进行的全基因组序列分析均无法检测到已知的鳞翅目活动基因（cry 1 ，cry 2和cry 9）毒素扫描仪工具或CryProcessor工具。因此，在草案基因组中蛋白质晶体的存在和对棉铃虫的毒性以及基因组草案中不存在任何已知的cry / vip / cyt类型基因表明它是新型的Bt分离株。对该基因组序列以及蛋白质测序的进一步研究将有助于理解造成其毒力的新因素，并且可能是粉红色棉铃虫抗虫性管理的有用工具。