To better understand the molecular basis of corpus luteum (CL) development and function RNA-Seq was utilized to identify differentially expressed genes (DEGs) in porcine CL during different physiological stages of the estrous cycle viz. early (EL), mid (ML), late (LL) and regressed (R) luteal. Stage wise comparisons obtained 717 (EL vs. ML), 568 (EL vs. LL), 527 (EL vs. R), 786 (ML vs. LL), 474 (ML vs. R) and 534 (LL vs. R) DEGs with log₂(FC) ≥1 and p < 0.05. The process of angiogenesis, steroidogenesis, signal transduction, translation, cell proliferation and tissue remodelling were significantly (p < 0.05) enriched in EL, ML and LL stages, where as apoptosis was most active in regressed stage. Pathway analysis revealed that most annotated genes were associated with lipid metabolism, translation, immune and endocrine system pathways depicting intra-luteal control of diverse CL function. The network analysis identified genes AR, FOS, CDKN1A, which were likely the novel hub genes regulating CL physiology.

为了更好地了解黄体 (CL) 发育和功能的分子基础，利用 RNA-Seq 在发情周期的不同生理阶段识别猪 CL 中的差异表达基因 (DEG)。早期 (EL)、中期 (ML)、晚期 (LL) 和退化 (R) 黄体。阶段性比较获得 717 (EL vs. ML)、568 (EL vs. LL)、527 (EL vs. R)、786 (ML vs. LL)、474 (ML vs. R) 和 534 (LL vs. R) ) log ₂ (FC) ≥1 且p的 DEG < 0.05。血管生成、类固醇生成、信号转导、翻译、细胞增殖和组织重塑的过程在EL、ML和LL阶段显着（p <0.05）富集，而细胞凋亡在退化阶段最为活跃。通路分析显示，大多数注释基因与脂质代谢、翻译、免疫和内分泌系统通路相关，这些通路描述了黄体内对不同 CL 功能的控制。网络分析确定了基因 AR、FOS、CDKN1A，它们可能是调节 CL 生理学的新中枢基因。