Laccase mediated delignification of lignocellulosic biomass generates phenolic compounds which normally inhibits the enzymatic hydrolysis of cellulose and suppresses the reaction rate drastically. To overcome the inhibitory effects and maximize productivity, pretreatment is requisite. This work proposes the extraction of phenolic compounds at the pretreatment stage to increase glucose yield in the enzymatic hydrolysis of lignocellulosic biomass-Bambusa Bamboo. In addition, recovery of lignin derived phenolic compounds is also targeted by adsorption followed by desorption. For adsorbent preparation, chemically modified cenospheres from waste coal fly ash and chitosan were cross-linked to form syntactic foam adsorbent. Adsorption and desorption studies were carried out on synthesized adsorbent using gallic acid as a phenolic equivalent. In our investigation, 40.31% delignification was achieved when the feed of the average particle size of 60 μm was operated in a batch reactor. The extraction of phenolic compounds significantly increases glucose yield and 1.4 times higher glucose yield was obtained for delignified plus extracted biomass than that of only delignified biomass. Syntactic foam adsorbent has shown excellent adsorption capacity for phenolic compounds with a rapid rate of adsorption in initial hours and exhibited maximum adsorption capacity as 1.07 mmol of gallic acid per gram of adsorbent. Thus, extraction of lignin derived phenolic inhibitors prior to enzymatic hydrolysis, and recovery of these phenolic compounds by adsorption can make the process more viable for the sustainable production of biofuels in the biorefinery.

漆酶介导的木质纤维素生物质的脱木质素作用产生酚类化合物，该酚类化合物通常抑制纤维素的酶促水解并极大地抑制反应速率。为了克服抑制作用并最大化生产率，必须进行预处理。这项工作建议在预处理阶段提取酚类化合物，以提高木质纤维素生物质-Bambusa Bamboo的酶水解中的葡萄糖产量。。另外，木质素衍生的酚类化合物的回收也通过吸附然后脱附来实现。为了制备吸附剂，将煤粉煤灰和壳聚糖中的化学改性的空心微珠进行交联以形成复合泡沫吸附剂。使用没食子酸作为酚当量对合成的吸附剂进行吸附和解吸研究。在我们的调查，40.31％的脱木素达到60时的平均粒径的进料 μm在间歇反应器中操作。酚类化合物的提取显着提高了葡萄糖产量，脱木质素和提取生物质的葡萄糖产量比仅脱木质素的生物质高1.4倍。合成泡沫吸附剂对酚类化合物的吸附能力极佳，在开始的几个小时内吸附速度很快，最大吸附能力为每克吸附剂1.07 mmol没食子酸。因此，在酶促水解之前提取木质素衍生的酚类抑制剂，以及通过吸附回收这些酚类化合物可使该方法对于在生物精炼厂中可持续生产生物燃料更加可行。