The measures currently used to minimize the spread of arboviruses, comprising dengue, Chikungunya, and Zika virus, involve controlling the size of population of the mosquito Aedes aegypti. However, the search for formulations containing new insecticides is gaining pace due to reports of mosquito populations showing resistance to commonly used compounds. In this study, tablets containing a protein fraction of Moringa oleifera seeds enriched in the WSMoL lectin, known to show larvicidal and ovicidal activities against A. aegypti, were developed. The compatibility between the fraction and the excipients used in obtaining the tablets was evaluated by thermogravimetry (TG), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) absorption spectroscopy. The larvicidal and ovicidal activities of the resulting tablets [5%, 10%, and 15% (w/w) of the fraction] were evaluated, as well as their effect on mosquito oviposition. Assays were also performed using a placebo tablet. According to the TG, DSC, and FTIR results, the protein composition of the fraction did not change when mixed with the components of the formulation. Tablets containing 10% and 15% WSMoL-rich fraction caused mortality of 42.5% and 95% of the larvae after 48 h, respectively, with larvae incubated with these tablets showing reduced acetylcholinesterase activity. All tablets inhibited egg hatching after 72 h (36–74%), and tablets containing 15% fraction were found to exert a repellent effect on oviposition. Our results show that the formulation developed in this study interfered with the life cycle of A. aegypti, and thus show potential for use in the control of this mosquito.

当前用于使包括登革热，基孔肯雅热和寨卡病毒在内的虫媒病毒的传播最小化的措施包括控制埃及伊蚊的种群规模。然而，由于有报道说蚊子对常用化合物具有抗性，因此寻找含有新型杀虫剂的制剂的步伐正在加快。在这项研究中，片剂含有辣木种子的蛋白质部分，富含WSMoL凝集素，已知具有抗埃及伊蚊的杀幼虫和杀卵活性。，被开发出来。通过热重法（TG），差示扫描量热法（DSC）和傅里叶变换红外光谱（FTIR）吸收光谱法评估级分和用于获得片剂的赋形剂之间的相容性。评价所得片剂的杀幼虫和杀卵活性[占级分的5％，10％和15％（w / w）]，以及它们对蚊子产卵的影响。还使用安慰剂片剂进行测定。根据TG，DSC和FTIR结果，该级分的蛋白质组成与配方成分混合后没有变化。含有10％和15％富含WSMoL的级分的片剂在48小时后分别导致幼虫的死亡率为42.5％和95％，与这些片剂一起孵育的幼虫显示出降低的乙酰胆碱酯酶活性。所有片剂均在72小时后抑制卵孵化（36–74％），并且发现含有15％的片剂对产卵具有驱避作用。我们的结果表明，本研究开发的制剂干扰了维生素的生命周期。埃及伊蚊，因此显示出可用于控制这种蚊子的潜力。