Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p < 0.05) decreased in the CP treated group relative to the control. However, there was an increase in the weight of the organs in the LLLE pretreated groups. The photomicrographs showed degenerative changes in the testicular tissues of the rats administered CP alone whereas the group pretreated with the LLLE showed amelioration induced by the CP. Our study revealed that CP treatment has deleterious effects on sperm parameters and testicular tissues and the accessory sex organs (Epididymis, prostate, seminal vesicles) of the rats. Oral administration of LLLE at 200 and 400 mg/kg bodyweight for 26 days conferred protective effects against testicular damage induced by CP. This study revealed that pretreatment with LLLE protected against CP-induced testicular toxicity.

中文翻译：

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披针草（Lophira lanceolata）保护顺铂对雄性Wistar大鼠睾丸和精子的损伤

化学疗法与男性不育有关。顺铂（CP）是一种抗肿瘤药，已成功用于治疗各种恶性肿瘤，但是，使用这种有效药物可能会引起氧化应激损伤，肾毒性，肝毒性和睾丸损伤。CP化疗与植物提取物的联合使用可减少毒性并增强药物的抗肿瘤功效。该研究的目的是确定披针叶叶提取物（LLLE）对CP诱导的雄性生殖器官毒性的保护作用。该研究是对30只（n = 30）雄性Wistar大鼠（Rattus norvegicus）进行的。将大鼠随机分为6组，每组5只。第1组（对照组）的大鼠经口口服蒸馏水。第2组的大鼠腹膜内给予5 mg / kg的CP（i。p）。第3组和第4组的大鼠按os LLLE的剂量分别以200和400 mg / kg体重的剂量给药，第5和第6组的大鼠以5 mg / kg体重的CP + LLLE的剂量按200和400 mg的剂量给药/ kg体重。结果显示，与对照组相比，仅用CP治疗的组精子参数显着下降，而在CP + LLLE组中，精子参数显着下降。与对照组相比，CP治疗组大鼠的体重和器官重量显着降低（p <0.05）。然而，LLLE预处理组的器官重量增加。显微照片显示，单独给予CP的大鼠睾丸组织的退行性改变，而用LLLE预处理的组则表现出CP引起的改善。我们的研究表明，CP处理对大鼠的精子参数，睾丸组织和附属性器官（Epididymis，前列腺，精囊）具有有害作用。口服LLLE 200和400 mg / kg体重持续26天可预防CP引起的睾丸损伤。这项研究表明，LLLE预处理可防止CP诱发的睾丸毒性。