Near Full-Length Genomic Characterization of 16 HIV-1 CRF01_AE Primary Isolates from Guangxi, China,AIDS Research and Human Retroviruses

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Near Full-Length Genomic Characterization of 16 HIV-1 CRF01_AE Primary Isolates from Guangxi, China
AIDS Research and Human Retroviruses ( IF 1.5 ) Pub Date : 2021-07-01 , DOI: 10.1089/aid.2020.0277
Guoxin Zhu _{1,

2} , Jingwan Han ₂ , Hanping Li ₂ , Yongjian Liu ₂ , Lei Jia ₂ , Tianyi Li ₂ , Xiaolin Wang ₂ , Jingyun Li ₂ , Shenghai Huang _{1,

3} , Lin Li ₂

Affiliation

Isolation and culture of human immunodeficiency virus (HIV) are an important basis for acquired immune deficiency syndrome (AIDS) etiology, immunology, drug screening, clinical treatment, and vaccine research. CRF01_AE is one of the predominant strains of HIV-1 in China. However, there are few HIV-1 CRF01_AE isolates that have been reported. In this study, 16 HIV-1 CRF01_AE strains from Guangxi, China, were isolated, and the near full-length genomes were reverse transcribed and amplified in two halves with the 1 kb overlapping region. The polymerase chain reaction products were sequenced directly. The phylogenetic analysis results showed that all of the 16 isolated strains were CRF01_AE recombinant form, and two clusters were set up in the phylogenetic tree. The tropic prediction of 16 strains showed that 2 isolates were CCR5 tropic, and the others are CXCR4 tropic. Eight of the isolated strains are drug resistant according to the genetic prediction. These 16 near full-length characterized CRF01_AE isolates obtained in this study will provide valuable genomic and phenotypic information on HIV-1 strains circulating in China for related researches.

更新日期：2021-07-07

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