Bioprinting of unmodified soft extracellular matrix into complex 3D structures has remained challenging to fabricate. Herein, we established a novel process for the printing of low-viscosity hydrogel by using a unique support technique to retain the structural integrity of the support structure. We demonstrated that this process of printing could be used for different types of hydrogel, ranging from fast crosslinking gelatin methacrylate to slow crosslinking collagen type I. In addition, we evaluated the biocompatibility of the process by observing the effects of the cytotoxicity of L929 and the functionality of the human umbilical vein endothelium primary cells after printing. The results show that the bioprinted construct provided excellent biocompatibility as well as supported cell growth and differentiation. Thus, this is a novel technique that can be potentially used to enhance the resolution of the extrusion-based bioprinter.

中文翻译：

---

通过模型支持生物墨水相互作用对低粘度细胞水凝胶进行高分辨率新型间接生物打印

将未修饰的软细胞外基质生物打印成复杂的 3D 结构仍然具有挑战性。在此，我们通过使用独特的支撑技术来保持支撑结构的结构完整性，建立了一种打印低粘度水凝胶的新工艺。我们证明了这种打印工艺可用于不同类型的水凝胶，从快速交联的甲基丙烯酸明胶到慢速交联的 I 型胶原蛋白。此外，我们通过观察 L929 的细胞毒性和打印后人脐静脉内皮原代细胞的功能。结果表明，生物打印构建体具有出色的生物相容性，并支持细胞生长和分化。因此，