Cervical carcinoma is the third most common cause of cancer in women with a significant challenge in clinical treatment. Human papillomavirus (HPV) is strongly responsible for cervical carcinoma. Here, we show the increased expression level of heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) in HPV-associated cervical cancer cells including HeLa, Caski, and SiHa cells, especially in HeLa cells. We provide the evidence that the expression of HNRNPA1 is closely related to HeLa cell proliferation, invasion, and migration. Emerging evidence show that histone modifications account for gene expression. Moreover, our results indicate that HNRNPA1 could be regulated by p300 through p300-mediated lysine crotonylation. Inhibition of p300 downregulated both the lysine crotonylation level and the HNRNPA1 expression. And p300-mediated lysine crotonylation participates in the regulation of HNRNPA1 on HeLa cell proliferation, invasion, and migration. Collectively, our study uncovers that p300-mediated lysine crotonylation enhances expression of HNRNPA1 to promote the proliferation, invasion, and migration of HeLa cells.

中文翻译：

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p300 催化的赖氨酸巴豆酰化通过异质核糖核蛋白 A1 促进 HeLa 细胞的增殖、侵袭和迁移

宫颈癌是女性癌症的第三大常见原因，临床治疗面临重大挑战。人乳头瘤病毒 (HPV) 是导致宫颈癌的主要原因。在这里，我们显示异质核糖核蛋白 A1 (HNRNPA1) 在 HPV 相关宫颈癌细胞（包括 HeLa、Caski 和 SiHa 细胞）中的表达水平增加，尤其是在 HeLa 细胞中。我们提供的证据表明 HNRNPA1 的表达与 HeLa 细胞增殖、侵袭和迁移密切相关。新出现的证据表明，组蛋白修饰是基因表达的原因。此外，我们的结果表明 HNRNPA1 可以通过 p300 介导的赖氨酸巴豆酰化受 p300 调节。p300 的抑制下调了赖氨酸巴豆酰化水平和 HNRNPA1 表达。p300 介导的赖氨酸巴豆酰化参与了 HNRNPA1 对 HeLa 细胞增殖、侵袭和迁移的调节。总的来说，我们的研究揭示了 p300 介导的赖氨酸巴豆酰化增强了 HNRNPA1 的表达，从而促进了 HeLa 细胞的增殖、侵袭和迁移。