ABSTRACT

GmGASA is the GASA gibberellin regulated cysteine-rich protein family. The expression of GmGASA is up-regulated by gibberellin, which is the longest plant hormone in plants playing vital roles in plant development. However, very few reports explaining the direct regulation of downstream genes by GASA gene are available. In the current study, the GmGASA32, a member of the GASA family affecting soybean height was identified. In the early stage, preliminary verification of the response of GmGASA32 to gibberellin through phenotypic experiment was done. The promoter activity analysis confirmed that GmGASA32 was induced by gibberellin. Subcellular localization showed that GmGASA32-GFP fusion protein enriched in the nucleus after gibberellin treatment. In order to confirm the function of GmGASA32 in the nucleus, we confirmed that the GASA domain in the C terminal of GmGASA32 can interact with GmCDC25 (cell cycle-associated protein) through the bimolecular fluorescence complementation (BiFC) assay.

摘要

GmGASA是GASA赤霉素调节的富含半胱氨酸的蛋白质家族。GmGASA的表达被赤霉素上调，赤霉素是植物中最长的植物激素，在植物发育中起着至关重要的作用。但是，很少有报道解释GASA基因对下游基因的直接调控。在当前的研究中，鉴定了影响大豆高度的GASA家族成员GmGASA32。在早期阶段，通过表型实验初步验证了GmGASA32对赤霉素的反应。启动子活性分析证实了GmGASA32是由赤霉素诱导的。亚细胞定位表明，赤霉素处理后，GmGASA32-GFP融合蛋白富集在细胞核中。为了确认GmGASA32在细胞核中的功能，我们证实了GmGASA32 C末端的GASA结构域可以通过双分子荧光互补（BiFC）分析与GmCDC25（细胞周期相关蛋白）相互作用。