Abstract

Genome-wide association studies (GWAS) have accelerated the identification of functional trait loci in cattle and identified single nucleotide polymorphisms (SNPs) in candidate genes associated with fertility and production traits in high milk yield dairy cattle. The fertility of Hanwoo (Korean native beef cattle) has declined after the adaptation of a selection program for high quantity and quality meat. However, there are few GWAS studies of fertility in beef cattle. We performed a genome-wide association study of 40 Korean native beef cattle heifers with imputed 770 K genotype and identified 12 significant SNPs within seven regions on three chromosomes (BTA 8, BTA 16 and BTA 24) associated with services per conception (SPC). Five SNPs were located in the ABCA1, BRINP3 and ESRRG genes, which are involved in early embryo development. In addition, 27 proximal genes were identified within 1 Mb of the candidate SNPs, which are involved in muscle cell differentiation and muscle structure development. However, we did not find any previously reported SNPs related to fertility in Holstein cows. Taken together, we identified SNPs associated with SPC and their proximal genes using gene-based analysis and the candidates were different from SNPs associated with subfertility of dairy cattle.

摘要

全基因组关联研究（GWAS）加快了牛功能性状基因座的鉴定，并在与高产奶牛的生育力和生产性状相关的候选基因中鉴定了单核苷酸多态性（SNP）。在对高品质和高品质肉类的选择计划进行调整之后，韩宇（韩国土特产牛）的繁殖力下降了。但是，很少有GWAS研究对肉牛的生育能力进行研究。我们对40个具有770 K基因型的韩国本土肉牛小母牛进行了全基因组关联研究，并在与每个概念服务（SPC）相关的三个染色体（BTA 8，BTA 16和BTA 24）的七个区域中确定了12个重要SNP。五个SNP位于ABCA1，BRINP3和ESRRG基因，与早期胚胎发育有关。此外，在候选SNP的1 Mb范围内鉴定出27个近端基因，这些基因与肌肉细胞分化和肌肉结构发育有关。但是，我们没有发现任何先前报道的与荷斯坦奶牛生育力有关的SNP。综上所述，我们使用基于基因的分析方法鉴定了与SPC及其近端基因相关的SNP，而这些候选物与与奶牛亚生育力相关的SNP有所不同。