Abstract

Mentha × Piperita L. or peppermint belonging to the family Lamiaceae (mint) is an important herb and useful in pharmaceutical, medicinal and cosmetic industries. In this study, the interactive effects of the foliar application of chitosan and inoculation of the rhizomes of peppermint seedlings with arbuscular mycorrhizal fungi (AMF) on growth and of the contents of menthol and balance of menthol/menthone of the essential oil from M × Piperita in a randomized complete block design (RCBD) were evaluated. The essential oil from the aerial parts (stem and leaves) of this herb was analyzed by GC-FID and GC/MS. Results indicated that the experimental treatments especially the inoculation of the peppermint plants with AMF influenced fresh and dry herbal weights and dry matter of the herb. The major constituents of the essential oil of M × Piperita were menthol and menthone belonging to oxygenated monoterpenes. The highest contents of essential oil yield, menthol and balance of menthol/menthone of the essential oil from M × Piperita were obtained from the peppermint plants under foliar spraying of chitosan and inoculation with AMF, however, the herbs under the foliar application of chitosan (both concentrations) without inoculation showed the highest percentages of menthone and limonene. The results indicated that elicitation of the peppermint with chitosan and inoculation of the peppermint plants with AMF has positive effects on dry herbal weight and secondary metabolites and can be improved the quantity and quality active substances of M × Piperita L.

摘要

属于唇形科（薄荷）的薄荷（Mentha×Piperita L.）或薄荷是一种重要的草药，可用于制药，医药和化妆品行业。本研究研究了叶面施用壳聚糖和接种丛枝菌根真菌（AMF）的薄荷幼苗的根茎对M×Piperita精油中薄荷醇的含量，薄荷醇和薄荷的生长和含量的相互作用。在随机完整块设计（RCBD）中进行了评估。通过GC-FID和GC / MS分析了该草药的地上部分（茎和叶）的精油。结果表明，实验处理尤其是AMF对薄荷植物的接种会影响新鲜和干燥草药的重量以及草药的干物质。M×Piperita精油的主要成分是薄荷脑和薄荷酮，属于氧化单萜。M×Piperita精油的最高含量，薄荷醇和薄荷油/薄荷酮的平衡含量最高壳聚糖是从薄荷植物叶面喷洒壳聚糖并用AMF接种获得的，但是，在未接种接种壳聚糖（两种浓度）的叶面施用的草药中，薄荷酮和and烯的含量最高。结果表明，壳聚糖诱导薄荷和AMF接种薄荷植物对干燥草本体重和次生代谢物有积极作用，可以改善M×Piperita L的活性和数量。