Abstract

This research aimed to investigate the effect of different extraction methods and chemical composition of the essential oil (EO) and solid-phase microextraction (SPME) from Campanula latifolia L. sub-sp. latifolia which is native to Turkey, with different medicinal properties. Phenolic components, antioxidant and enzyme inhibitory effects of essential oil (EO), n-hexane, acetonitrile, methanol and water extracts of C. latifolia subsp. latifolia were also studied. The volatile components of the EO, SPME and SPME of n-hexane extracts, obtained from the Campanula latifolia L. subsp. latifolia, were analyzed by GC-FID/MS. A total of 32, 31 and 36 compounds were identified comprising 99.8 %, 99.9 % and 94.4 % of the SPME, SPME of n-hexane extract and EO of C. latifolia subsp. latifolia, respectively. The major compounds identified in the SPME and SPME of n-hexane extract were limonene (44.0 %) and 1,2,3-trimethyl benzene (27.4 %), respectively. Whereas the EO of Campanula latifolia subsp. latifolia was rich with capro aldehyde (23.1 %), nonanal (9.3 %), 2(E)-hexenal (6.8 %), benzene acetaldehyde (6.8 %), E-β-caryophyllene (4.4 %) and E-β-ionone (3.3 %). The phenolic content of extracts was analyzed by RP-HPLC. Results revealed the sinapic, benzoic, syringic and p-coumaric acids as major constituents. Antioxidant activities (DPPH, PRAP and FRAP) and enzyme inhibitory (tyrosinase, acetylcholinesterase (AChE) and α-glucosidase) effects of the EO, n-hexane, acetonitrile, methanol and water extracts obtained from C. latifolia subsp. latifolia were investigated. The acetonitrile extract, among the other extracts of C. latifolia subsp. latifolia, was found to have the highest antioxidant activity and inhibitory effect for all tested enzymes due to its high phenolic content.

摘要

本研究旨在调查不同提取方法和化学成分对桔梗子油菜（Campanula latifolia L.sp.）的精油（EO）和固相微萃取（SPME）的影响。latifolia，原产于土耳其，具有不同的药用特性。酚组分，抗氧化剂和精油（EO），的酶抑制作用Ñ己烷，乙腈，甲醇和水的提取物C. latifolia的亚种。还研究了阔叶植物。该EO的挥发性组分，和固相微萃取的SPME Ñ己烷提取物，从所获得的风铃latifolia的L.亚种。阔叶植物通过GC-FID / MS分析。共有32，31和36的化合物被鉴定，其包括99.8％，99.9％和SPME的94.4％，的SPME Ñ己烷提取物的EO和C. latifolia的亚种。阔叶分别。在SPME和SPME鉴定的主要化合物ñ -己烷提取物分别为柠檬烯（44.0％）和1,2,3-三甲基苯（27.4％）。而风铃草的EO的亚种。latifoli a富含己醛（23.1％），壬醛（9.3％），2（E）-己醛（6.8％），苯乙醛（6.8％），E -β-石竹烯（4.4％）和E-β-紫罗兰酮（3.3％）。提取物中的酚含量通过RP-HPLC分析。结果表明正弦酸，苯甲酸，丁香酸和对香豆酸是主要成分。抗氧化活性（DPPH，PRAP和FRAP）和酶抑制（酪氨酸酶，乙酰胆碱酯酶和α葡糖苷酶）的EO的效果，Ñ己烷，乙腈，从获得的甲醇和水提取物C. latifolia的亚种。调查了阔叶植物。乙腈提取物，C。latifolia亚种的其他提取物。由于其高酚含量，发现对所有测试的酶来说，阔叶连翘具有最高的抗氧化活性和抑制作用。