Common vetch (Vicia sativa subsp. sativa L.) is a self-pollinating annual forage legume with high nutritional value. However, pod shattering can cause severe yield loss at maturity and therefore is a significant trait for crop improvement. Our previous studies indicated that the ventral suture is critical to pod shattering in common vetch. To better understand the anatomy of the ventral suture, we evaluated eight shatter-susceptible and 18 shatter-resistant accessions for pod dehiscing characteristics over three consecutive years by comprehensively comparing the vascular bundle anatomical structure of the ventral suture. Four traits, specifically vascular bundle cell volume, bundle cap slope, single vascular bundle width, and external valve margin cells, were significantly different between the shatter-susceptible and -resistant accessions. We found that the shatter-resistant common vetches had a larger vascular bundle cell volume and bundle cap slope, an increased single vascular bundle width, and external valve margin cells that were significantly thicker than those of shatter-susceptible accessions. All of the shatter-susceptible pods had clear abscission layers. On the basis of these data, we propose a seesaw model of pod shattering in common vetch. Our morphological traits provide a novel insight into the pod shattering mechanism of common vetch, which will be valuable for breeding shatter-resistant legumes.

中文翻译：

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抗破碎和易破碎的普通紫云英种质中荚腹缝合线的显着细胞差异

野豌豆 ( Vicia sativa subsp. sativaL.) 是一种自花授粉的一年生牧草豆科植物，具有很高的营养价值。然而，荚果破碎会导致成熟时严重的产量损失，因此是作物改良的一个重要性状。我们之前的研究表明，腹侧缝合线对于普通紫云英中的豆荚破碎至关重要。为了更好地了解腹缝线的解剖结构，我们通过综合比较腹缝线的维管束解剖结构，连续三年评估了 8 个易碎和 18 个抗碎种质的豆荚开裂特征。四个特征，特别是维管束细胞体积、束帽斜率、单维管束宽度和外部瓣膜边缘细胞，在易破碎和抗破碎的种质之间存在显着差异。我们发现抗碎裂的普通vetches具有更大的维管束细胞体积和束帽斜率，增加的单维管束宽度和外部瓣膜边缘细胞明显厚于易碎的加入物。所有易碎的豆荚都有清晰的脱落层。在这些数据的基础上，我们提出了一种普通野豌豆荚破碎的跷跷板模型。我们的形态特征为了解普通紫云英的荚果破碎机制提供了新的见解，这对于培育抗破碎豆类很有价值。所有易碎的豆荚都有清晰的脱落层。在这些数据的基础上，我们提出了一种普通野豌豆荚破碎的跷跷板模型。我们的形态特征为了解普通紫云英的荚果破碎机制提供了新的见解，这对于培育抗破碎豆类很有价值。所有易碎的豆荚都有清晰的脱落层。在这些数据的基础上，我们提出了一种普通野豌豆荚破碎的跷跷板模型。我们的形态特征为了解普通紫云英的荚果破碎机制提供了新的见解，这对于培育抗破碎豆类很有价值。