BRITTLE PLANT1 is required for normal cell wall composition and mechanical strength in rice,Journal of Integrative Plant Biology

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BRITTLE PLANT1 is required for normal cell wall composition and mechanical strength in rice
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-12-08 , DOI: 10.1111/jipb.13050
Yuanyan Zhang ₁ , Yihua Wang ₁ , Chunming Wang ₁ , Carsten Rautengarten ₂ , Erchao Duan ₁ , Jianping Zhu ₁ , Xiaopin Zhu ₁ , Jie Lei ₁ , Chao Peng ₁ , Yunlong Wang ₁ , Xuan Teng ₁ , Yunlu Tian ₁ , Xi Liu ₁ , Joshua L Heazlewood ₂ , Aimin Wu ₃ , Jianmin Wan _{1,

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Affiliation

A series of nucleotide sugar interconversion enzymes (NSEs) generate the activated sugar donors required for biosynthesis of cell wall matrix polysaccharides and glycoproteins. UDP‐glucose 4‐epimerases (UGEs) are NSEs that function in the interconversion of UDP‐glucose (UDP‐Glc) and UDP‐galactose (UDP‐Gal). The roles of UDP‐glucose 4‐epimerases in monocots remain unclear due to redundancy in the pathways. Here, we report a brittle plant (bp1) rice mutant that exhibits brittle leaves and culms at all growth stages. The mutant culms had reduced levels of rhamnogalacturonan I, homogalacturonan, and arabinogalactan proteins. Moreover, the mutant had altered contents of uronic acids, neutral noncellulosic monosaccharides, and cellulose. Map‐based cloning demonstrated that OsBP1 encodes a UDP‐glucose 4‐epimerase (OsUGE2), a cytosolic protein. We also show that BP1 can form homo‐ and hetero‐protein complexes with other UGE family members and with UDP‐galactose transporters 2 (OsUGT2) and 3 (OsUGT3), which may facilitate the channeling of Gal to polysaccharides and proteoglycans. Our results demonstrate that BP1 participates in regulating the sugar composition and structure of rice cell walls.

中文翻译：

BRITTTLE PLANT1 是水稻正常细胞壁组成和机械强度所必需的

一系列核苷酸糖互变酶 (NSE) 生成细胞壁基质多糖和糖蛋白生物合成所需的活化糖供体。UDP-葡萄糖 4-差向异构酶 (UGE) 是在 UDP-葡萄糖 (UDP-Glc) 和 UDP-半乳糖 (UDP-Gal) 相互转化中起作用的 NSE。由于途径中的冗余，UDP-葡萄糖4-差向异构酶在单子叶植物中的作用仍不清楚。在这里，我们报告了一种脆性植物( bp1 ) 水稻突变体，它在所有生长阶段都表现出脆弱的叶子和秆。突变株的鼠李半乳糖醛酸 I、同型半乳糖醛酸聚糖和阿拉伯半乳糖醛酸蛋白的水平降低。此外，突变体的糖醛酸、中性非纤维素单糖和纤维素的含量发生了变化。基于图谱的克隆证明OsBP1编码 UDP-葡萄糖 4-差向异构酶 (OsUGE2)，一种胞质蛋白。我们还表明，BP1 可以与其他 UGE 家族成员以及 UDP-半乳糖转运蛋白 2 (OsUGT2) 和 3 (OsUGT3) 形成同源和异源蛋白复合物，这可能促进 Gal 向多糖和蛋白聚糖的通道。我们的结果表明BP1参与调节水稻细胞壁的糖组成和结构。

更新日期：2020-12-08

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