Soybean seed includes various bioactive substances. Also, they contain a variety of antinutritional factors including lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin, and 7S α' subunit proteins. The genetic removal of these proteins will improve the nutritional value of soybean seed. The objective of this research was to breed new soybean with tetra recessive alleles (lox1lox2lox3/lox1lox2lox3‐ti/ti‐le/le‐cgy1/cgy1) for lipoxygenase, KTI, lectin, and 7S α' subunit proteins. Seven parents were used to breed tetra null strain. SDS‐PAGE and Western blot analysis were used to determine the presence or absence of lipoxygenase, 7S α' subunit, KTI, and lectin proteins in mature seed. Tetra null soybean line has a purple flower, determinate growth habit, tan pod, and yellow seed coat colour. Stem height of the breeding line was 62.3 cm. The 100‐seed weight of the breeding line was 27.1 g and yield (t/ha) was 2.84. This is the first soybean strain with lox1lox2lox3/lox1lox2lox3‐ti/ti‐le/le‐cgy1/cgy1 genotype (absence of lipoxygenase, KTI, lectin, and 7S α' subunit proteins).

中文翻译：

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脂肪氧化酶，kunitz胰蛋白酶抑制剂，凝集素和7Sα'亚基蛋白的四空大豆（Glycine max）的选育

大豆种子包含各种生物活性物质。此外，它们还包含多种抗营养因子，包括脂氧合酶，Kunitz胰蛋白酶抑制剂（KTI），凝集素和7Sα '亚基蛋白。这些蛋白质的遗传去除将改善大豆种子的营养价值。这项研究的目的是培育具有四个隐性等位基因（lox1lox2lox3 / lox1lox2lox3 - ti / ti - le / le - cgy1 / cgy1）的大豆，用于脂肪氧化酶，KTI，凝集素和7Sα '亚基蛋白。使用七个亲本繁殖四无效株。SDS-PAGE和蛋白质印迹分析用于确定成熟种子中是否存在脂加氧酶，7Sα '亚基，KTI和凝集素蛋白。利乐零大豆品系具有紫色花朵，确定的生长习性，棕褐色和黄色种皮颜色。繁殖线的茎高为62.3厘米。育种线的100粒重为27.1 g，单产（t / ha）为2.84。这是第一个大豆菌株lox1lox2lox3 / lox1lox2lox3 - TI / TI -乐/乐- cgy1 / cgy1基因型（无脂氧合酶，KTI，凝集素，和7S的α'亚基蛋白）。