The set of all intra- and intermolecular interactions, collectively known as the interactome, is currently an unmet challenge for any analytical method, but if measured, could provide unparalleled insight on molecular function in living systems. Developments and applications of chemical cross-linking and high-performance mass spectrometry technologies are beginning to reveal details on how proteins interact in cells and how protein conformations and interactions inside cells change with phenotype or during drug treatment or other perturbations. A major contributor to these advances is Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) technology and its implementation with accurate mass measurements on cross-linked peptide-pair precursor and fragment ions to enable improved identification methods. However, these applications place increased demands on mass spectrometer performance in terms of high-resolution spectral acquisition rates for on-line MSⁿ experiments. Moreover, FT-ICR-MS also offers unique opportunities to develop and implement parallel ICR cells for multiplexed signal acquisition and the potential to greatly advance accurate mass acquisition rates for interactome studies. This review highlights our efforts to exploit accurate mass FT-ICR-MS technologies with chemical cross-linking and developments being pursued to realize parallel MS array capabilities that will further advance visualization of the interactome.

中文翻译：

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FT-ICR-MS 在相互作用组研究中的应用和进展


所有分子内和分子间相互作用的集合，统称为相互作用组，目前对任何分析方法来说都是一个未解决的挑战，但如果进行测量，可以为生命系统中的分子功能提供无与伦比的见解。化学交联和高性能质谱技术的发展和应用开始揭示蛋白质如何在细胞中相互作用以及蛋白质构象和细胞内相互作用如何随表型或在药物治疗或其他扰动期间变化的细节。这些进步的主要贡献者是傅里叶变换离子回旋共振质谱 (FT-ICR-MS) 技术及其对交联肽对前体和碎片离子进行精确质量测量的实施，以改进识别方法。然而，这些应用对在线 MS ⁿ实验的高分辨率光谱采集率方面的质谱仪性能提出了更高的要求。此外，FT-ICR-MS 还提供了开发和实施用于多重信号采集的并行 ICR 细胞的独特机会，并有可能大大提高相互作用组研究的精确质量采集率。本综述强调了我们在利用精确质量 FT-ICR-MS 技术与化学交联方面所做的努力，以及为实现并行 MS 阵列功能而进行的开发，这将进一步推进相互作用组的可视化。