This study obtained the bioactivity potentials (antioxidant, anti-inflammatory and enzyme inhibitory) of ethanol (EtOH), water (H2O) and chloroform (CHCl3) extracts of G. applanatum, G. lucidum, G. pfeifferi and G. resinaceum as well as their mycochemical profile: the total content of phenolics (TP) and sugars (TS) and LC-MS/MS detection of phenolics. LC-MS/MS profile showed that p-hydroxybenzoic and protocatechuic acids were mostly founded. The highest ABTS and DPPH activities were detected in polar G. applanatum extracts (159.84±0.59 mg TE/g d.w., IC50=0.85±0.30 mg/mL, respectively), while G. resinaceum CHCl3 extract was the most potent in NO assay (IC50=41.21±0.18-81.89±0.81 mg/mL). The highest TP and TS were generally determined in G. applanatum EtOH extracts. Enzyme inhibitory effects were determined in H2O extracts. The most powerful anti-inflammatory activities showed generally CHCl3 extracts. These results suggest that analyzed species are a promising source of bioactive compounds and may be considered as candidates for new food supplements or drug formulations.

中文翻译：

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抗炎、抗氧化和酶抑制活性与选定的本土灵芝属的真菌化学特征相关。来自巴尔干地区（塞尔维亚）

本研究还获得了 G. applanatum、G. 灵芝、G. pfeifferi 和 G.resinaceum 的乙醇 (EtOH)、水 (H2O) 和氯仿 (CHCl3) 提取物的生物活性潜力（抗氧化、抗炎和酶抑制）作为它们的真菌化学特征：酚类物质 (TP) 和糖类 (TS) 的总含量以及酚类物质的 LC-MS/MS 检测。LC-MS/MS 谱显示主要是对羟基苯甲酸和原儿茶酸。在极性 G. applanatum 提取物中检测到最高的 ABTS 和 DPPH 活性（分别为 159.84±0.59 mg TE/g dw，IC50=0.85±0.30 mg/mL），而 G.resinaceum CHCl3 提取物在 NO 测定中最有效（ IC50=41.21±0.18-81.89±0.81 毫克/毫升）。最高的 TP 和 TS 通常在 G. applanatum EtOH 提取物中测定。在 H2O 提取物中测定酶抑制作用。最强大的抗炎活性通常显示出 CHCl3 提取物。这些结果表明，所分析的物种是生物活性化合物的有希望的来源，可以被视为新食品补充剂或药物配方的候选者。