Dynamics of Chemical Diversity During Co‐cultures: An Integrative Time‐scale Metabolomics of Fungal Endophytes Cophinforma mamane and Fusarium solani,Chemistry & Biodiversity

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Dynamics of Chemical Diversity During Co‐cultures: An Integrative Time‐scale Metabolomics of Fungal Endophytes Cophinforma mamane and Fusarium solani
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-01-07 , DOI: 10.1002/cbdv.202000672
Asih Triastuti _{1,

2} , Mohamed Haddad ₁ , Fatima Barakat ₁ , Kember Mejia ₃ , Gabriel Rabouille ₁ , Nicolas Fabre ₁ , Carlos Amasifuen ₄ , Patricia Jargeat ₅ , Marieke Vansteelandt ₁

Affiliation

A rapid and efficient metabolomic study of Cophinforma mamane and Fusarium solani co-cultivation in time-series based analysis was developed to study metabolome variations during their fungal interactions. The fungal metabolomes were studied through the integration of four metabolomic tools: MS-DIAL, a chromatographic deconvolution of liquid-chromatography-mass spectrometry (LC-MS); MS-FINDER, a structure-elucidation program with a wide range metabolome database; GNPS, an effective method to organize MS/MS fragmentation spectra, and MetaboAnalyst, a comprehensive web application for metabolomic data analysis and interpretation. Co-cultures of C. mamane and F. solani induced different patterns of metabolite production over 10 days of incubation and induced production of three de novo compounds not occurring in monocultures. These results emphasize that co-culture in time-frame analysis is an interesting method to unravel hidden metabolome in the investigation of fungal chemodiversity.

中文翻译：

共培养过程中化学多样性的动态：真菌内生菌 Cophinforma mamane 和 Fusarium solani 的综合时间尺度代谢组学

在基于时间序列的分析中对 Cophinforma mamane 和 Fusarium solani 共培养进行了快速有效的代谢组学研究，以研究它们在真菌相互作用过程中的代谢组变化。通过整合四种代谢组学工具来研究真菌代谢组：MS-DIAL，一种液相-色谱-质谱 (LC-MS) 的色谱解卷积；MS-FINDER，具有广泛代谢组数据库的结构解析程序；GNPS，一种组织 MS/MS 碎裂谱的有效方法，以及 MetaboAnalyst，一种用于代谢组学数据分析和解释的综合网络应用程序。C. mamane 和 F. solani 的共培养物在 10 天的孵化过程中诱导了不同的代谢物产生模式，并诱导了三种在单一培养中不存在的从头化合物的产生。

更新日期：2021-01-07

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