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Calcium-hydrogen sulfide crosstalk during K+-deficient NaCl stress operates through regulation of Na+/H+ antiport and antioxidative defense system in mung bean roots
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2020-12-08 , DOI: 10.1016/j.plaphy.2020.11.055
M. Nasir Khan , Manzer H. Siddiqui , Soumya Mukherjee , Saud Alamri , Abdullah A. Al-Amri , Qasi D. Alsubaie , Bander M.A. Al-Munqedhi , Hayssam M. Ali

Present investigation reports the role of calcium (Ca2+) and hydrogen sulfide (H2S) crosstalk associated with Vigna radiata seedlings subjected to K+ deficient conditions under short-term (24 h) and long-term (72 h) NaCl stress. Perusal of the data reveals that under short-term NaCl stress an initial decline in K+ level led to the elevation in Ca2+ and H2S levels along with improvement in antioxidant system and reduction in reactive oxygen species (ROS) production. Under long-term NaCl stress a further decline in K+ content was deleterious that led to a lower K+/Na+ ratio. This was followed by reduction in antioxidant system along with excessive accumulation of ROS and methylglyoxal content, and increased membrane damage. However, supplementation of the seedling roots with Ca2+ enhanced biosynthesis of H2S through enhancing cysteine pool. The present findings suggest that synergistic action of Ca2+ and H2S induced the activity of H+-ATPase that created H+ gradient which in turn induced Na+/H+ antiport system that accelerated K+ influx and Na+ efflux. All of these together contributed to a higher K+/Na+ ratio, activation of antioxidative defense system, and maintenance of redox homeostasis and membrane integrity in Ca2+-supplemented stressed seedlings. Role of Ca2+ and H2S in the regulation of Na+/H+ antiport system was validated by the use of sodium orthovanadate (plasma membrane H+-ATPase inhibitor), tetraethylammonium chloride (K+ channel blocker), and amiloride (Na+/H+ antiporter inhibitor). Application of Ca2+-chelator EGTA (ethylene glycol-bis(b-aminoethylether)-N,N,Nʹ,Nʹ-tetraacetic acid) and H2S scavenger hypotaurine abolished the effect of Ca2+, suggesting the involvement of Ca2+ and H2S in the alleviation of NaCl stress. Moreover, use of EGTA and HT also substantiates the downstream functioning of H2S during Ca2+-mediated regulation of plant adaptive responses to NaCl stress. To sum up, present findings reveal the association of Ca2+ and H2S signaling in the regulation of ion homeostasis and antioxidant defense during K+-deficient NaCl stress.



中文翻译:

ķ期间钙硫化氢串扰+缺陷型NaCl胁迫通过钠的调节操作+ / H +在绿豆根反向转运和抗氧化防御系统

本调查报告钙(Ca的作用2+)和硫化氢(H 2 S)串扰相关的绿豆进行至K苗+短期(24小时)和长期(72小时)盐胁迫下缺乏条件。数据的统计显示,在短期NaCl胁迫下,K +含量的初始下降导致Ca 2+和H 2 S含量升高,同时抗氧化剂系统得到改善,活性氧(ROS)产量降低。在长期的NaCl胁迫下,K +含量进一步下降是有害的,导致K + / Na +降低。比。其次是减少抗氧化剂系统,以及过多的ROS和甲基乙二醛含量积聚,并增加膜损伤。然而,通过增强半胱氨酸池,用Ca 2+补充幼苗根可以增强H 2 S的生物合成。目前的发现表明,Ca 2+和H 2 S的协同作用诱导了H + -ATPase的活性,从而产生了H +梯度,进而诱导了Na + / H +反转运系统,从而加速了K +内流和Na +外排。所有这些共同导致更高的K + / Na +Ca 2+胁迫的幼苗中的比例,抗氧化防御系统的活化以及氧化还原稳态和膜完整性的维持。通过使用原钒酸钠(质膜H + -ATPase抑制剂),氯化四乙铵(K +通道阻滞剂)和阿米洛利(以下简称Amir)验证了Ca 2+和H 2 S在Na + / H +反向转运系统调节中的作用。Na + / H +反向转运抑制剂)。Ca 2 +-螯合剂EGTA(乙二醇-双(b-氨基乙基醚)-N,N,Nʹ,Nʹ-四乙酸)和H 2的应用S清除剂低牛磺酸消除了Ca 2+的作用,表明Ca 2+和H 2 S参与了NaCl胁迫的缓解。此外,EGTA和HT的使用还证实了Ca 2+介导的植物对NaCl胁迫的适应性调节过程中H 2 S的下游功能。综上所述,目前的发现揭示了Ca 2+和H 2 S信号传导在缺钾+ NaCl胁迫期间调节离子稳态和抗氧化防御的关联。

更新日期:2020-12-29
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