Abstract In this research, a solid-phase denitrification reactor (SPDR) packed with corrugated paperboard was introduced into the recirculating aquaculture system (RAS) of Litopenaeus vannamei for nitrate removal. Results showed that 82.88% of the nitrogen was removed from the culture water with the denitrification rate of 0.74 mg L−1·d−1 and the concentration of nitrate was controlled at 2.31 ± 1.00 mg L−1. High throughput sequencing analysis showed that the corrugated paperboard was degraded by Lachnospiraceae, Saprospiraceae, Exiguobacterium, Mesoflavibacter, etc. to provide carbon source for denitrifying bacteria, including Rhizobiaceae, Rhodobacteraceae, Bacillus, Nitratireductor, Sphingobium, Marinicella, Desulfovibrio, Marinobacter, and Labrenzia. The abundance of the functional genes amoA, nirK, and nosZ indicated that nitrogen can be efficiently converted into N2 in the SPDR.

中文翻译：

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循环水养殖系统中纤维素载体生物反应器的脱氮性能和微生物多样性

摘要 本研究将瓦楞纸板固相反硝化反应器（SPDR）引入南美白对虾循环水养殖系统（RAS）中去除硝酸盐。结果表明，培养水中脱氮率为82.88%，反硝化率为0.74 mg L-1·d-1，硝酸盐浓度控制在2.31±1.00 mg L-1。高通量测序分析表明，瓦楞纸板被毛螺菌科、腐生菌科、细杆菌、中黄杆菌等降解，为根瘤菌科、红杆菌科、芽孢杆菌、硝化还原菌、鞘氨醇、海藻属、脱硫杆菌、脱硫菌、蓝藻等反硝化细菌提供碳源。功能基因 amoA、nirK、