Certain fungal endophytes improve plant resistance to biotic stresses in forest trees. In this study, three stem fungal endophytes belonging to classes Cystobasidiomycetes, Eurotiomycetes and Dothideomycetes were selected from 210 isolates for their potential as enhancers of Ulmus minor resistance to Ophiostoma novo-ulmi. We evaluated phenotypic traits of these endophytes that could be beneficial for inhibiting O. novo-ulmi in the host plant. Under in vitro conditions, the Dothideomycetous isolate YCB36 strongly inhibited O. novo-ulmi growth, released antipathogenic VOCs, chitinases and siderophores, and overlapped with the pathogen in nutrient utilization patterns. These functional traits could explain the 40% reduction in leaf wilting due to O. novo-ulmi in elm trees pre-inoculated with this endophyte. Ulmus minor trees inoculated with this endophyte showed increased leaf stomatal conductance and higher concentrations of flavonoids and total phenolic compounds in xylem tissues, suggesting induction of defence metabolism.

某些真菌内生菌可提高植物对林木中生物胁迫的抗性。在这项研究中，三茎属于类囊担菌纲，散囊菌纲和座囊菌纲，从210株挑选适合自己的作为增强潜在的内生真菌小榆耐蛇口壳NOVO-榆。我们评估了这些内生植物的表型性状，这些性状可能对抑制寄主植物中的O. novo-ulmi有益。下在体外条件下，分离物Dothideomycetous YCB36强烈抑制O. NOVO-螨生长，释放出抗病原性VOC，几丁质酶和铁载体，并在养分利用模式中与病原体重叠。这些功能性状可以解释在预先接种了该内生菌的榆树中，由于新乌尔姆氏菌引起的枯萎率降低了40％。接种该内生菌的小榆树显示出叶片气孔导度增加，木质部组织中的类黄酮和总酚类化合物浓度更高，表明诱导了防御代谢。