The aim of this work was to compare the yield and fatty acids profile of Moringa oleifera oils obtained with supercritical fluids extraction (SFE) and solvent extraction. Seed proteins from residual defatted flour were characterized according to the Osborne's fractionation and by shotgun proteomics in gel approach. SFE showed higher yield (35%) than solvent extraction method (20%). Fatty acids profile were similar being oleic acid the compound of the highest abundance followed by myristic, palmitic, stearic, and behenic acids. A unique profile of seed storage proteins (SSPs) was observed. 2S albumins, chitin binding protein-2, morintide, protein disulfure-isomerases, and proteins related with photorespiration were identified. Present results showed that SFE is an efficient green method to obtain high oil yields from Moringa seeds. Moringa SSPs profile has been characterized for the first time, which gives a whole panorama of protein seed composition and its solubility properties.

这项工作的目的是比较辣木的产量和脂肪酸谱通过超临界流体萃取（SFE）和溶剂萃取获得的石油。残留脱脂面粉中的种子蛋白根据Osborne的分级分离和shot弹枪蛋白质组学在凝胶法中进行表征。SFE显示出比溶剂萃取方法（20％）更高的产率（35％）。脂肪酸谱相似，其中油酸是丰度最高的化合物，其次是肉豆蔻酸，棕榈酸，硬脂酸和山hen酸。观察到种子存储蛋白（SSPs）的独特特征。鉴定了2S白蛋白，几丁质结合蛋白2，morintide，蛋白二硫键异构酶和与光呼吸有关的蛋白。目前的结果表明，SFE是一种从辣木种子获得高油产量的有效绿色方法。辣木SSP的配置文件首次被表征，