Carboxylesterases (CEs) are key enzymes which catalyse the hydrolysis reactions of multiple xenobiotics and endogenous ester moieties. Given their growing interest in the context of marine pollution and biomonitoring, this study focused on the in vitro sensitivity of marine invertebrate CEs to some pesticides, pharmaceuticals, personal care products and plastic additives to assess their potential interaction on this enzymatic system and its suitability as biomarkers. Three bivalves, one gastropod and two crustaceans were used and CEs were quantified following current protocols set for mammalian models. Four substrates were screened for CEs determination and to test their adequacy in the hepatic fraction measures of the selected invertebrates. Two commercial recombinant human isoforms (hCE1 and hCE2) were also included for methodological validation. Among the invertebrates, mussels were revealed as the most sensitive to xenobiotic exposures while gastropods were the least as well as with particular substrate-specific preferences. Among chemicals of environmental concern, the plastic additive tetrabromobisphenol A displayed the highest CE-inhibitory capacity in all species. Since plastic additives easily breakdown from the polymer and may accumulate and metabolise in marine biota, their interaction with the CE key metabolic/detoxification processes may have consequences in invertebrate’s physiology, affect bioaccumulation and therefore trophic web transfer and, ultimately, human health as shellfish consumers.

羧基酯酶（CEs）是催化多种异源生物和内源性酯部分水解反应的关键酶。考虑到他们对海洋污染和生物监测的兴趣日益浓厚，本研究着重于体外海洋无脊椎动物CE对某些农药，药物，个人护理产品和塑料添加剂的敏感性，以评估其在该酶体系上的潜在相互作用及其作为生物标记物的适用性。使用三个双壳类动物，一个腹足动物和两个甲壳类动物，并按照当前为哺乳动物模型设定的方案对CE进行定量。筛选了四种底物用于CE的测定，并测试了它们在所选无脊椎动物肝分数测量中的适用性。还包括两种商业重组人同工型（hCE1和hCE2）用于方法论验证。在无脊椎动物中，贻贝被揭示为对异种生物接触最敏感，而腹足类动物则最少，并且具有特定的底物特异性偏好。在涉及环境的化学品中，塑料添加剂四溴双酚A在所有物种中均表现出最高的CE抑制能力。由于塑料添加剂很容易从聚合物中分解出来，并可能在海洋生物区中积累和代谢，因此它们与CE关键代谢/解毒过程的相互作用可能会对无脊椎动物的生理产生影响，影响生物蓄积，进而影响营养网的转移，最终影响贝类消费者的健康。 。