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Phytochemical screening and antioxidant, analgesic, and anthelmintic effect of ethanolic extract of Merremia umbellate stems
Clinical Phytoscience Pub Date : 2020-12-07 , DOI: 10.1186/s40816-020-00232-6
Mst Nazmun Nahar , Amit Kumar Acharzo , Md Sohanur Rahaman , Iffat Ara Zabeen , Shariful Haque , Md Amirul Islam

Merremia umbellata subsp. orientalis (Hallier f.), commonly known as Sapussunda in Bengali, is used in folk medicine for the treatment of different diseases such as helminthiasis, rheumatism, fever, wounds, burns, sores, management of pain due to cut etc. The present study was carried out to evaluate the antioxidant, analgesic and anthelmintic activities of ethanolic extract of stems of Merremia umbellate (ESMU). Phytochemical investigation was carried by using standard chemical test as described in literatures. In vitro free radical scavenging activity of ethanolic extract was quantitatively estimated using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals scavenging assay. Total phenolic and tannin content were spectrophotometrically determined by Folin Ciocalteu reagent whereas the flavonoid was determined by aluminum chloride colorimetric assay. Acetic acid induced writhing method and hot plate method, using Swiss albino mice, were used to investigate the analgesic effect of ESMU whereas in-vitro anthelmintic activity was evaluated against Haemonchus contortus (Nematode) of cattle. Phytochemical screening revealed that the ESMU contain reducing sugar, alkaloids, flavonoids, tannins, gums, steroid, xanthoproteins, glycosides and acidic compound. In DPPH free radical scavenging assay, the extract showed scavenging potential with IC50 value of 161.81 μg/mL. Total phenolics, tannin and flavonoid content of crude extract were found to be 87.4 mg GAE/gm, 68.2 mg GAE /gm and 64.27 mg QE/gm respectively. Significant (P < 0.001) analgesic effect was observed in acetic acid induced writhing method at both doses 250 and 500 mg/kg. Similar effect was found in hot plate method that measures antinociceptive effect in response to heat stimuli. The ESMU also exhibited significant (P < 0.001) anthelmintic activity in a concentration dependent manner. The paralysis time and time for death were recorded as 9.30, 8.62 and 7.65 min and 19.58, 18.82, and 16.43 min respectively at a concentration of 25, 50 and 100 mg/mL respectively. Based on the results obtained in this study clearly strengthen the traditional uses of M. umbellate stems as antioxidant, antinociceptive and anthelmintic. Therefore, this result suggested that the stems of Merremia umbellata might be a potential source of useful bioactive compounds.

中文翻译:

伞形Merremia茎的乙醇提取物的植物化学筛选和抗氧化,止痛和驱虫作用

伞形亚目 Orientalis(Hallier f。),在孟加拉语中通常被称为Sapussunda,在民间医学中用于治疗各种疾病,例如蠕虫病,风湿病,发烧,伤口,烧伤,疮,割伤引起的疼痛等。进行了评价伞形Merremia伞形(ESMU)乙醇提取物的抗氧化,止痛和驱虫活性。通过使用文献中描述的标准化学测试进行植物化学研究。使用DPPH(2,2-二苯基-1-picylhydrazyl)自由基清除测定法定量评估乙醇提取物的体外自由基清除活性。总酚和单宁含量用Folin Ciocalteu试剂分光光度法测定,而类黄酮则用氯化铝比色法测定。使用瑞士的白化病小鼠,通过乙酸诱导的扭体法和热板法研究ESMU的镇痛作用,同时评估了体外驱虫活性对牛的Haconchus contortus(Nematode)的影响。植物化学筛选显示,ESMU含有还原糖,生物碱,类黄酮,单宁,树胶,类固醇,黄体蛋白,糖苷和酸性化合物。在DPPH自由基清除试验中,提取物具有清除潜力,IC50值为161.81μg/ mL。粗提物中的总酚,单宁和类黄酮含量分别为87.4 mg GAE / gm,68.2 mg GAE / gm和64.27 mg QE / gm。在250和500 mg / kg的剂量下,乙酸诱导的扭体法均观察到了明显的镇痛作用(P <0.001)。在热板法中发现了类似的效果,该方法可测量对热刺激的镇痛效果。ESMU还以浓度依赖性方式表现出显着的(P <0.001)驱虫活性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。001)在250和500 mg / kg剂量的乙酸诱导的扭体法中均观察到镇痛作用。在热板法中发现了类似的效果,该方法可测量对热刺激的镇痛效果。ESMU还以浓度依赖性方式表现出显着的(P <0.001)驱虫活性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。001)在250和500 mg / kg剂量的乙酸诱导的扭体法中均观察到镇痛作用。在热板法中发现了类似的效果,该方法可测量对热刺激的镇痛效果。ESMU还以浓度依赖性方式表现出显着的(P <0.001)驱虫活性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。在热板法中发现了类似的效果,该方法可测量对热刺激的镇痛效果。ESMU还以浓度依赖性方式表现出显着的(P <0.001)驱虫活性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。在热板法中发现了类似的效果,该方法可测量对热刺激的镇痛效果。ESMU还以浓度依赖性方式表现出显着的(P <0.001)驱虫活性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。001)驱虫活性呈浓度依赖性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间,以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。001)驱虫活性呈浓度依赖性。分别以25、50和100 mg / mL的浓度分别记录了9.30、8.62和7.65分钟的麻痹时间和死亡时间以及19.58、18.82和16.43分钟的死亡时间。根据本研究获得的结果,可以清楚地证明伞形藻茎的传统用途是作为抗氧化剂,抗伤害感受药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。伞形茎为抗氧化剂,镇痛药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。伞形茎为抗氧化剂,镇痛药和驱虫药。因此,该结果表明伞形Merremia umbellata的茎可能是有用的生物活性化合物的潜在来源。
更新日期:2020-12-07
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