Lipoproteins at aberrant levels are known to play a role in cardiovascular disease. The metabolite of the insecticide dichlorodiphenyltrichloroethane (DDT), p,p’-dichlorodiphenyldichloroethylene (p,p’-DDE), physically associates with lipids and accumulates in adipose tissue. Little is known about which lipoproteins associate with p,p’-DDE. An association between p,p’-DDE exposure and altered levels of circulating lipids was assessed in a large human cohort using a detailed analysis of lipoprotein content. Plasma samples were collected from the subset of 75-year old Swedes in the Prospective Investigation of the Vasculature of Uppsala Seniors (PIVUS) cohort who were not prescribed lipid lowering medication (n = 571). p,p’-DDE concentrations in plasma were measured using high-throughput solid phase extraction and gas chromatography-high resolution mass spectrometry. Analysis of plasma lipoprotein content was performed with nuclear magnetic resonance spectroscopy. Detectable levels of p,p’-DDE were found in the plasma samples of all subjects. Elevated p,p’-DDE levels were associated with increased concentrations of lipoproteins of all diameters, with the exception of high density lipoprotein (HDL) of diameters between 14.3 nm–10.9 nm. Of the lipoprotein constituents, triglycerides were most uniformly associated with elevated p,p’-DDE across lipoproteins. p,p’-DDE was furthermore associated with apolipoprotein B, but not apolipoprotein A1. The positive associations observed between each lipoprotein class and elevated p,p’-DDE support previous data suggesting that p,p’-DDE interacts with lipoproteins within plasma. It is speculated that both physio-chemical and biological mechanisms may explain why p,p’-DDE does not uniformly associate with lipids across lipoproteins.

中文翻译：

---

通过脂蛋白含量分析确定老年人群中 p,p'-DDE 水平与脂蛋白及其亚类血浆水平之间的关联

已知异常水平的脂蛋白在心血管疾病中发挥作用。杀虫剂二氯二苯基三氯乙烷 (DDT) 的代谢产物对，对'-二氯二苯基二氯乙烯 (p,p'-DDE) 在物理上与脂质结合并在脂肪组织中积聚。关于哪些脂蛋白与 p,p'-DDE 相关，人们知之甚少。通过对脂蛋白含量的详细分析，在大型人群中评估了 p,p'-DDE 暴露与循环脂质水平改变之间的关联。血浆样本采集自乌普萨拉老年人脉管系统前瞻性调查 (PIVUS) 队列中未服用降脂药物的 75 岁瑞典人 (n = 571)。p, 使用高通量固相萃取和气相色谱-高分辨率质谱法测量血浆中的 p'-DDE 浓度。用核磁共振波谱分析血浆脂蛋白含量。在所有受试者的血浆样本中均发现了可检测水平的 p,p'-DDE。p,p'-DDE 水平升高与所有直径的脂蛋白浓度升高相关，但直径在 14.3 nm–10.9 nm 之间的高密度脂蛋白 (HDL) 除外。在脂蛋白成分中，甘油三酯与脂蛋白中 p,p'-DDE 升高的相关性最为一致。p,p'-DDE 还与载脂蛋白 B 相关，但与载脂蛋白 A1 无关。每种脂蛋白类别与升高的 p,p'-DDE 之间观察到的正相关性支持了先前的数据，表明 p, p'-DDE 与血浆内的脂蛋白相互作用。据推测，物理化学和生物学机制可以解释为什么 p,p'-DDE 不与脂蛋白中的脂质一致地结合。