Cellulase, due to its massive applicability, has been used in various industrial processes such as biofuels (bioethanol, triphasic biomethanation), agricultural and plant waste management, chiral separation, and ligand binding studies. The finding of a novel cellulase-producing bacterium will benefit the industries, which rely on yeast to produce cellulase in fermentation technology, because bacteria can easily be manipulated and fermented cost-effectively. Cellulase enzyme-secreting bacteria were isolated from different regions of the world’s largest mangrove forests, Sundarbans in Bangladesh. Biochemical, morphological, and 16S rRNA identification protocol was followed to precisely characterize the bacterial strains. We have determined that the strain T2-D2 (Bacillus sp.), E1-PT (Pseudomonas sp.), and D1-PT (Pseudomonas sp.) showed maximum endoglycolytic and strain C1-BT (Bacillus sp.), E1-BT (Bacillus sp.), and T-4 (E) showed relatively higher exoglycolytic activity during the test. So, it can be easily cultured at a normal temperature (97.7–99.5 °F). On the one hand, T2-D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) have shown the highest growth rate at pH 7 as it was neither acidic nor basic. It was concluded that the strain T2-D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) would be our target cellulolytic strains wherein the experimental isolates belonged to the Enterobacteriaceae, Psuedomonacea, Bacillacea, and Morganellacea family.

中文翻译：

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孟加拉国最大的红树林（Sundarbans）中的纤维素分解（内切和外切葡聚糖酶）细菌的分子表征

纤维素酶由于其广泛的适用性，已用于各种工业过程中，例如生物燃料（生物乙醇，三相生物甲烷化），农业和植物废物管理，手性分离和配体结合研究。新型纤维素酶生产细菌的发现将有利于工业，该行业依靠酵母在发酵技术中生产纤维素酶，因为细菌可以很容易地以成本效益的方式进行操作和发酵。纤维素酶分泌细菌是从世界上最大的红树林，孟加拉国的桑达尔班斯的不同地区分离出来的。遵循生化，形态学和16S rRNA鉴定规程，以精确表征细菌菌株。我们已经确定菌株T2-D2（芽孢杆菌属），E1-PT（假单胞菌属）和D1-PT（假单胞菌属。）显示出最大的内切糖酵解能力，而菌株C1-BT（芽孢杆菌属），E1-BT（芽孢杆菌属）和T-4（E）在测试过程中表现出相对较高的外切糖活性。因此，可以轻松地在常温（97.7–99.5°F）下进行培养。一方面，T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）在pH 7时显示出最高的生长速率，因为它既不是酸性也不是碱性的。结论是，菌株T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）将成为我们的目标纤维素分解菌株，其中实验分离株属于肠杆菌科，假单胞菌属，杆菌属和摩根氏菌科。T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）在pH 7下显示出最高的生长速率，因为它既不是酸性也不是碱性。结论是，菌株T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）将成为我们的目标纤维素分解菌株，其中实验分离株属于肠杆菌科，假单胞菌属，杆菌属和摩根氏菌科。T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）在pH 7时显示出最高的生长速率，因为它既不是酸性也不是碱性。结论是，菌株T2-D2（芽孢杆菌属）和E1-PT（假单胞菌属）将是我们的目标纤维素分解菌株，其中实验分离株属于肠杆菌科，假单胞菌属，杆菌属和摩根氏菌科。