The increasing concentration of livestock farms results in large amounts of waste production and the need for their management. The study of anaerobic digestion (AD) technology, under mesophilic conditions, applied to pig slurry is of the upmost importance for biogas recovery and sanitised digestate, contributing to a circular economy. The assessment of the effects of a feast/famine regime on biogas and biomethane (bio-CH₄) yield with different feeding frequencies was performed. The evaluation was made in regards to three scenarios: the first is based on daily feeding (FR1); in the second, the feeding occurs once every two days (FR2); and in the third, the feeding happens once every three days (FR3). The results demonstrate that the biogas and methane yield increased by 34% and 37% between FR1 and FR3. The stability inside the reactor was maintained since specific loading energetic rate values did not exceed the recommended limit (0.4 d⁻¹). It was also possible to conclude that the AD technology was efficient to sanitise the pig slurry, with the count of Escherichia coli going from 1 × 10⁵ colony-forming units (CFU) g⁻¹ to less than 100 CFU g⁻¹, meeting the legal requirements for agricultural valorisation. The total anaerobic mesophile plate counts were significantly (p < 0.1) reduced from feeding to digestate, and the plate counts of Clostridia were significantly (p < 0.05) increased, reflecting the changes in the composition of the microbiota. The increasing yield in bio-CH₄ in accordance with Clostridium counts suggests this genus as a positive microbiological key indicator of the AD performance.

畜牧场的日益集中导致大量的废物产生以及对其进行管理的需要。在中温条件下对猪粪进行厌氧消化（AD）技术的研究对于沼气的回收和消化液的消毒至关重要，这有助于实现循环经济。评估节日/饥荒制度对沼气和沼气的影响（bio-CH ₄）以不同的进料频率进行产量。对以下三种情况进行了评估：第一种是基于每日进食量（FR1）；第二种是基于日粮。在第二次，每两天喂一次（FR2）；第三，每三天喂一次（FR3）。结果表明，FR1和FR3之间的沼气和甲烷产率分别提高了34％和37％。由于特定的负载能率值未超过建议的极限（0.4 d ^-1），因此维持了反应器内部的稳定性。也有可能得出结论，AD技术可以有效地对猪粪进行消毒，大肠杆菌的数量从1×10 ⁵ 菌落形成单位（CFU）g ^-1降至小于100 CFU g^-1，满足农业物价评估的法律要求。 从进食到消化，总的厌氧嗜温菌平板数显着减少（p <0.1），梭菌的平板数显着增加（p  <0.05），反映了微生物群组成的变化。根据梭菌计数，生物CH ₄产量的增加表明该属是AD性能的积极微生物关键指标。