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Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-12-07 , DOI: 10.1021/acs.biomac.0c01317
Sebastian Raja 1, 2 , Ahmed E I Hamouda 2 , Marcelo A S de Toledo 2 , Chaolei Hu 3 , Marcela P Bernardo 1, 2 , Carmen Schalla 2 , Liliane S F Leite 1 , Eva Miriam Buhl 4 , Stephan Dreschers 5 , Andrij Pich 3 , Martin Zenke 2 , Luiz H C Mattoso 1 , Antonio Sechi 2
Affiliation  

Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells.

中文翻译:

功能化纤维素纳米晶体用于细胞标记和生物成像

纤维素纳米晶体(CNC)是独特且很有前途的天然纳米材料,可以通过酸水解从天然纤维素纤维中提取。在这项研究中,我们通过对基于聚乙二醇化生物素和per二酰亚胺(PDI)的近红外有机染料进行共价束缚,开发了化学修饰的CNC衍生物,并评估了它们对包括J774A.1巨噬细胞,NIH-3T3成纤维细胞在内的不同细胞系的标记和成像的适用性,HeLa腺癌细胞和原代鼠树突状细胞。与类似的市售染料相比,PDI标记的CNC在长时间的恒定和高强度照明下显示出优异的光稳定性。所有CNC衍生物对所有细胞类型均表现出优异的细胞相容性,并以剂量​​依赖性方式有效标记细胞。此外,CNCs被有效地内化并定位在核周周围区域的细胞质中。因此,我们的发现证明了这些新的CNC衍生物对于各种细胞系和原代细胞的标记,成像和长期跟踪的适用性。
更新日期:2021-02-08
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