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Phase separation of SERRATE drives dicing body assembly and promotes miRNA processing in Arabidopsis
Nature Cell Biology ( IF 17.3 ) Pub Date : 2020-12-07 , DOI: 10.1038/s41556-020-00606-5
Dongqi Xie 1, 2 , Min Chen 1, 2 , Jinrong Niu 1, 2 , Liang Wang 2, 3 , Yan Li 1, 2 , Xiaofeng Fang 1 , Pilong Li 2, 3 , Yijun Qi 1, 2, 3
Affiliation  

MicroRNA (miRNA) production entails the step-wise processing of primary miRNAs (pri-miRNAs) into precursor miRNAs (pre-miRNAs) and miRNA/* duplexes by Dicing complexes containing DCL1, HYL1 and SE, which are localized in nuclear dicing bodies (D-bodies)1,2. Here, we show that D-bodies are phase-separated condensates. SE forms droplets and drives DCL1, HYL1 and pri/pre-miRNAs into the droplets in vitro, and mutation of SE abrogates the formation of D-bodies in vivo, which indicates that D-bodies arise through SE-mediated phase separation. Disruption of SE phase separation greatly reduces its activity in promoting miRNA processing both in vitro and in vivo. We further show that pre-miRNAs are processed into miRNA/* duplexes in the droplets and, after processing, miRNA/* duplexes are bound by HYL1 and released from the droplets. Our findings provide evidence that efficient miRNA processing depends on the SE-phase-separation-mediated formation of D-bodies and suggest a paradigm that the products made in phase-separated condensates can be shipped out for subsequent processes.



中文翻译:

SERRATE 的相分离驱动切割体组装并促进拟南芥中的 miRNA 加工

微小 RNA (miRNA) 的产生需要通过包含 DCL1、HYL1 和 SE 的切割复合物将初级 miRNA (pri-miRNA) 逐步加工成前体 miRNA (pre-miRNA) 和 miRNA/* 双链体,这些复合物位于核切割体中( D 体)1,2. 在这里,我们表明 D 体是相分离的凝聚态。SE 在体外形成液滴并驱动 DCL1、HYL1 和 pri/pre-miRNA 进入液滴,SE 的突变消除了体内 D 体的形成,这表明 D 体是通过 SE 介导的相分离产生的。SE 相分离的破坏大大降低了其在体外和体内促进 miRNA 加工的活性。我们进一步表明,pre-miRNA 在液滴中被加工成 miRNA/* 双链体,并且在加工后,miRNA/* 双链体被 HYL1 结合并从液滴中释放出来。我们的研究结果证明,有效的 miRNA 加工取决于 SE 相分离介导的 D 体形成,并提出了一种范例,即在相分离的凝聚物中制成的产品可以运出用于后续过程。

更新日期:2020-12-07
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