Abstract

The effect of Kigelia africana on mitochondrial membrane permeability transition has not been explored. In this study, the effect of a solvent fraction of Kigelia africana leaf extract on mitochondrial membrane permeability transition of rat brain and liver was evaluated. A methanol extract of K. africana leaves was fractionated into different solvents by vacuum liquid chromatography and following preliminary screening, the dichloromethane:ethylacetate (1:1) fraction was selected for further assays. Constituent phytochemicals in the fraction were revealed by thin-layer chromatography and further purification was carried out to characterize the compounds. Brain and liver mitochondria were isolated and used for mitochondrial membrane permeability transition and adenosine triphosphatase assays. Exogenous Ca²⁺ and Al³⁺ were used to trigger the mitochondrial membrane permeability transition opening. Physicochemical properties revealed by thin-layer chromatography showed that the isolated compounds were flavonoids. The extract inhibited mitochondrial membrane permeability transition opening in the presence and absence of triggering agents in brain and liver mitochondria. It also inhibited mitochondrial lipid peroxidation and adenosine triphosphatase activity. These results suggest that the extract can limit the rate of apoptosis via inhibition of mitochondrial membrane permeability transition which is pivotal to the mitochondrial apoptotic pathway and is an important therapeutic target in some pathological conditions.

摘要

尚未探索Kigelia Africana对线粒体膜通透性转变的影响。在这项研究中，评估了非洲菊叶提取物溶剂部分对大鼠脑和肝脏线粒体膜通透性转变的影响。K.africana的甲醇提取物通过真空液相色谱法将叶子分馏到不同的溶剂中，经过初步筛选，选择二氯甲烷:乙酸乙酯 (1:1) 部分进行进一步分析。通过薄层色谱法揭示组分中的植物化学成分，并进行进一步纯化以表征化合物。脑和肝线粒体被分离并用于线粒体膜通透性转换和腺苷三磷酸酶测定。外源 Ca ²⁺和 Al ³⁺用于触发线粒体膜通透性转变开放。薄层色谱法显示的物理化学性质表明，分离的化合物是类黄酮。在脑和肝线粒体中存在和不存在触发剂的情况下，该提取物抑制线粒体膜通透性转变开放。它还抑制线粒体脂质过氧化和腺苷三磷酸酶活性。这些结果表明，该提取物可以通过抑制线粒体膜通透性转变来限制细胞凋亡率，这对线粒体凋亡途径至关重要，并且是某些病理条件下的重要治疗靶点。