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Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-12-07 , DOI: 10.1111/jipb.13049 Mingzhou Yu 1 , Mingming Wu 1 , Yulong Ren 2 , Yihua Wang 1 , Jingfang Li 1 , Cailin Lei 2 , Yinglun Sun 1 , Xiuhao Bao 1 , Hongming Wu 1 , Hang Yang 1 , Tian Pan 1 , Yongfei Wang 1 , Ruonan Jing 1 , Mengyuan Yan 2 , Houda Zhang 2 , Lei Zhao 2 , Zhichao Zhao 2 , Xin Zhang 2 , Xiuping Guo 2 , Zhijun Cheng 2 , Bing Yang 1 , Ling Jiang 1 , Jianmin Wan 1, 2
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-12-07 , DOI: 10.1111/jipb.13049 Mingzhou Yu 1 , Mingming Wu 1 , Yulong Ren 2 , Yihua Wang 1 , Jingfang Li 1 , Cailin Lei 2 , Yinglun Sun 1 , Xiuhao Bao 1 , Hongming Wu 1 , Hang Yang 1 , Tian Pan 1 , Yongfei Wang 1 , Ruonan Jing 1 , Mengyuan Yan 2 , Houda Zhang 2 , Lei Zhao 2 , Zhichao Zhao 2 , Xin Zhang 2 , Xiuping Guo 2 , Zhijun Cheng 2 , Bing Yang 1 , Ling Jiang 1 , Jianmin Wan 1, 2
Affiliation
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Pentatricopeptide repeat (PPR) proteins, composing one of the largest protein families in plants, are involved in RNA binding and regulation of organelle RNA metabolism at the post‐transcriptional level. Although several PPR proteins have been implicated in endosperm development in rice (Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18 (flo18) with pleiotropic defects in both reproductive and vegetative development. Map‐based cloning and complementation tests showed that FLO18 encodes a mitochondrion‐targeted P‐type PPR protein with 15 PPR motifs. Mitochondrial function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5′‐end processing of mitochondrial nad5 transcripts encoding subunit 5 of nicotinamide adenine dinucleotide hydrogenase. These results suggested that FLO18 is involved in 5′‐end processing of nad5 messenger RNA and plays an important role in mitochondrial function and endosperm development.
中文翻译：
Rice FLOURY ENDSPERM 18 编码线粒体 nad5 信使 RNA 和胚乳发育 5' 加工所需的五肽重复蛋白
五肽重复序列 (PPR) 蛋白是植物中最大的蛋白质家族之一，参与转录后水平的 RNA 结合和细胞器 RNA 代谢的调节。尽管几种 PPR 蛋白与水稻 ( Oryza sativa ) 的胚乳发育有关，但许多 PPR的分子功能仍不清楚。在这里，我们鉴定了一种名为粉状胚乳 18 ( flo18 )的水稻胚乳突变体，其在生殖和营养发育方面均存在多效性缺陷。基于图谱的克隆和互补测试表明FLO18编码具有 15 个 PPR 基序的线粒体靶向 P 型 PPR 蛋白。线粒体功能在flo18 中被破坏突变体，如线粒体电子传递链中复合物 I 组装减少和线粒体形态改变所证明的那样。FLO18功能的丧失导致线粒体nad5转录物编码烟酰胺腺嘌呤二核苷酸氢化酶亚基 5 的 5 ' 端加工缺陷。这些结果表明FLO18参与了nad5信使 RNA 的5' 端加工，并在线粒体功能和胚乳发育中起重要作用。
更新日期：2020-12-07
中文翻译：
Rice FLOURY ENDSPERM 18 编码线粒体 nad5 信使 RNA 和胚乳发育 5' 加工所需的五肽重复蛋白
五肽重复序列 (PPR) 蛋白是植物中最大的蛋白质家族之一，参与转录后水平的 RNA 结合和细胞器 RNA 代谢的调节。尽管几种 PPR 蛋白与水稻 ( Oryza sativa ) 的胚乳发育有关，但许多 PPR的分子功能仍不清楚。在这里，我们鉴定了一种名为粉状胚乳 18 ( flo18 )的水稻胚乳突变体，其在生殖和营养发育方面均存在多效性缺陷。基于图谱的克隆和互补测试表明FLO18编码具有 15 个 PPR 基序的线粒体靶向 P 型 PPR 蛋白。线粒体功能在flo18 中被破坏突变体，如线粒体电子传递链中复合物 I 组装减少和线粒体形态改变所证明的那样。FLO18功能的丧失导致线粒体nad5转录物编码烟酰胺腺嘌呤二核苷酸氢化酶亚基 5 的 5 ' 端加工缺陷。这些结果表明FLO18参与了nad5信使 RNA 的5' 端加工，并在线粒体功能和胚乳发育中起重要作用。
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