An in vitro assay approach to investigate the potential impact of different doping agents on the steroid profile,Drug Testing and Analysis

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An in vitro assay approach to investigate the potential impact of different doping agents on the steroid profile
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2020-12-07 , DOI: 10.1002/dta.2991
Thomas Piper ₁ , Sonja Heimbach ₂ , Martin Adamczewski ₂ , Mario Thevis _{1,

3}

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The steroid profile, that is, the urinary concentrations and concentration ratios of selected steroids, is used in sports drug testing to detect the misuse of endogenous steroids such as testosterone. Since several years, not only population-based thresholds are applied but also the steroid profile is monitored via the Athlete Biological Passport whereby the individual reference ranges derived from multiple test results of the same athlete are compared to population-based thresholds. In order to maintain a high probative force of the passport, samples collected or analyzed under suboptimal conditions should not be included in the longitudinal review. This applies to biologically affected or degraded samples and to samples excluded owing to the presence of other substances potentially (or evidently) altering the steroid profile. Nineteen different doping agents comprising anabolic steroids, selective androgen receptor modulators, selective estrogen receptor modulators, ibutamoren, and tibolone were investigated for their effect on the steroid profile using an androgen receptor activation test, an androgen receptor binding assay, an aromatase assay, and a steroidogenesis assay. The in vitro tests were coupled with well-established liquid chromatography/mass spectrometry-based analytical approaches and for a subset of steroidal analytes by gas chromatography/mass spectrometry. The variety of tests employed should produce a comprehensive data set to better understand how a compound under investigation may impact the steroid profile. Although our data set may allow an estimate of whether or not a substance will have an impact on the overall steroid metabolism, predicting which parameter in particular may be influenced remains difficult.

中文翻译：

一种体外检测方法，用于研究不同掺杂剂对类固醇谱的潜在影响

类固醇概况，即所选类固醇的尿浓度和浓度比，用于运动药物测试，以检测内源性类固醇（如睾酮）的滥用。几年以来，不仅应用了基于人群的阈值，而且还通过运动员生物护照监测了类固醇分布，由此将从同一运动员的多项测试结果得出的个人参考范围与基于人群的阈值进行比较。为了保持护照的高证明力，纵向审查不应包括在次优条件下收集或分析的样本。这适用于受生物影响或降解的样品，以及由于存在可能（或明显）改变类固醇分布的其他物质而被排除的样品。使用雄激素受体激活试验、雄激素受体结合试验、芳香酶试验和芳香化酶试验研究了 19 种不同的兴奋剂，包括合成代谢类固醇、选择性雄激素受体调节剂、选择性雌激素受体调节剂、伊布莫伦和替勃龙。类固醇生成测定。体外测试与完善的基于液相色谱/质谱的分析方法相结合，并通过气相色谱/质谱分析了一部分甾体分析物。所采用的各种测试应产生一个全面的数据集，以更好地了解正在研究的化合物如何影响类固醇谱。尽管我们的数据集可以估计某种物质是否会对整体类固醇代谢产生影响，

更新日期：2020-12-07

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