Plasma technology has emerged as a promising tool in medicine that, however, requires not only efficacy but also toxicological assessments. Traditional cell culture systems are fast and economical, but they lack in vivo relevance; however, rodent models are highly complex and necessitate extended facilities. Zebrafish larvae bridge this gap, and many larvae can be analyzed in well plates in a single run, giving results in 1–2 days. Using the kINPen, we found plasma exposure to reduce hedging rates and viability in a dose‐dependent manner, accompanied with an increase in reactive oxygen species and a decrease of glutathione in plasma‐treated fish. Modest growth alterations were also observed. Altogether, zebrafish larvae constitute a fast, reliable, and relevant model for testing the toxicity of plasma sources.

中文翻译：

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斑马鱼幼虫作为血浆药物的毒性模型

血浆技术已经成为一种有前途的医学工具，但是，它不仅需要疗效，而且还需要进行毒理学评估。传统的细胞培养系统既快速又经济，但是它们缺乏体内相关性。但是，啮齿动物模型非常复杂，需要扩展设施。斑马鱼幼虫弥合了这一鸿沟，可以一次运行在孔板中分析许多幼虫，只需1-2天即可得出结果。使用kINPen，我们发现血浆暴露以剂量依赖的方式降低套期保值率和生存能力，同时伴随血浆处理的鱼中活性氧种类的增加和谷胱甘肽的减少。还观察到适度的生长变化。总之，斑马鱼幼虫构成了一种快速，可靠且相关的模型，用于测试血浆来源的毒性。