Chemical exchange rotation transfer imaging of phosphocreatine in muscle,NMR in Biomedicine

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Chemical exchange rotation transfer imaging of phosphocreatine in muscle
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-12-07 , DOI: 10.1002/nbm.4437
Zhongliang Zu _{1,

2} , Eugene C Lin _{1,

2} , Elizabeth A Louie _{1,

2} , Xiaoyu Jiang _{1,

2} , Christopher L Lankford _{1,

2} , Bruce Damon _{1,

2,

3,

4} , Mark D Does _{1,

2,

3} , John C Gore _{1,

2,

3,

4,

5} , Daniel F Gochberg _{1,

2,

5}

Affiliation

In chemical exchange saturation transfer (CEST) imaging, the signal at 2.6 ppm from the water resonance in muscle has been assigned to phosphocreatine (PCr). However, this signal has limited specificity for PCr since the signal is also sensitive to exchange with protein and macromolecular protons when using some conventional quantification methods, and will vary with changes in the water longitudinal relaxation rate. Correcting for these effects while maintaining reasonable acquisition times is challenging. As an alternative approach to overcome these problems, here we evaluate chemical exchange rotation transfer (CERT) imaging of PCr in muscle at 9.4 T. Specifically, the CERT metric, AREX_double,cpw at 2.6 ppm, was measured in solutions containing the main muscle metabolites, in tissue homogenates with controlled PCr content, and in vivo in rat leg muscles. PCr dominates CERT metrics around 2.6 ppm (although with nontrivial confounding baseline contributions), indicating that CERT is well‐suited to PCr specific imaging, and has the added benefit of requiring a relatively small number of acquisitions.

中文翻译：

肌肉中磷酸肌酸的化学交换旋转转移成像

在化学交换饱和转移 (CEST) 成像中，来自肌肉水共振的 2.6 ppm 信号已被分配给磷酸肌酸 (PCr)。然而，该信号对 PCr 的特异性有限，因为在使用一些常规定量方法时，该信号对与蛋白质和大分子质子的交换也很敏感，并且会随着水纵向弛豫率的变化而变化。在保持合理的采集时间的同时纠正这些影响具有挑战性。作为克服这些问题的替代方法，我们在这里评估了 9.4 T 下肌肉中 PCr 的化学交换旋转转移 (CERT) 成像。具体来说，CERT 指标 AREX _double,cpw在 2.6 ppm 下是在含有主要肌肉的溶液中测量的代谢物，在具有受控 PCr 含量的组织匀浆中，以及体内大鼠腿部肌肉中。 PCr 在 2.6 ppm 左右主导 CERT 指标（尽管具有重要的混杂基线贡献），这表明 CERT 非常适合 PCr 特定成像，并且具有需要相对少量采集的额外好处。

更新日期：2021-01-04

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