Micronucleus Test Reveals Genotoxic Effects in Bats Associated with Agricultural Activity,Environmental Toxicology and Chemistry

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Micronucleus Test Reveals Genotoxic Effects in Bats Associated with Agricultural Activity
Environmental Toxicology and Chemistry ( IF 3.6 ) Pub Date : 2020-12-07 , DOI: 10.1002/etc.4907
Natalia Sandoval-Herrera _{1,

2} , Jesusa Paz Castillo ₂ , L Gerardo Herrera Montalvo ₃ , Kenneth C Welch _{1,

2}

Affiliation

Bats play a vital role in our ecosystems and economies as natural pest‐control agents, seed dispersers, and pollinators. Agricultural intensification, however, can impact bats foraging near crops, affecting the ecosystem services they provide. Exposure to pesticides, for example, may induce chromosome breakage or missegregation that can result in micronucleus formation. Detection of micronuclei is a simple, inexpensive, and relatively minimally invasive technique commonly used to evaluate chemical genotoxicity but rarely applied to assess wildlife genotoxic effects. We evaluated the suitability of the micronucleus test as a biomarker of genotoxicity for biomonitoring field studies in bats. We collected blood samples from insectivorous bats roosting in caves surrounded by different levels of disturbance (agriculture, human settlements) in Colima and Jalisco, west central Mexico. Then, we examined the frequency of micronucleus inclusions in erythrocytes using differentially stained blood smears. Bats from caves surrounded by proportionately more (53%) land used for agriculture and irrigated year‐round had higher micronucleus frequency than bats from a less disturbed site (15% agriculture). We conclude that the micronucleus test is a sensitive method to evaluate genotoxic effects in free‐ranging bats and could provide a useful biomarker for evaluating risk of exposure in wild populations. Environ Toxicol Chem 2021;40:202–207. © 2020 SETAC

中文翻译：

微核试验揭示了与农业活动相关的蝙蝠的基因毒性效应

蝙蝠作为天然害虫控制剂、种子传播者和传粉者，在我们的生态系统和经济中发挥着至关重要的作用。然而，农业集约化会影响蝙蝠在作物附近觅食，从而影响它们提供的生态系统服务。例如，接触农药可能会导致染色体断裂或错误分离，从而导致微核形成。微核检测是一种简单、廉价且相对微创的技术，通常用于评估化学遗传毒性，但很少用于评估野生动物的遗传毒性效应。我们评估了微核试验作为蝙蝠生物监测现场研究遗传毒性生物标志物的适用性。我们从栖息在洞穴中的食虫蝙蝠采集血液样本，这些蝙蝠被不同程度的干扰（农业、人类住区）在墨西哥中西部的科利马州和哈利斯科州。然后，我们使用差异染色的血涂片检查了红细胞中微核内含物的频率。与来自较少受到干扰的地点（15% 的农业）的蝙蝠相比，来自洞穴中被更多（53%）用于农业和全年灌溉的土地包围的蝙蝠具有更高的微核频率。我们得出的结论是，微核试验是一种评估自由放养蝙蝠遗传毒性效应的敏感方法，可以为评估野生种群暴露风险提供有用的生物标志物。与来自较少受到干扰的地点（15% 的农业）的蝙蝠相比，来自洞穴中被更多（53%）用于农业和全年灌溉的土地包围的蝙蝠具有更高的微核频率。我们得出的结论是，微核试验是一种评估自由放养蝙蝠遗传毒性效应的敏感方法，可以为评估野生种群暴露风险提供有用的生物标志物。与来自较少受到干扰的地点（15% 的农业）的蝙蝠相比，来自洞穴中被更多（53%）用于农业和全年灌溉的土地包围的蝙蝠具有更高的微核频率。我们得出的结论是，微核试验是一种评估自由放养蝙蝠遗传毒性效应的敏感方法，可以为评估野生种群暴露风险提供有用的生物标志物。环境毒理学化学2021；40：202-207。© 2020 SETAC

更新日期：2020-12-23

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