当前位置: X-MOL 学术J. Photochem. Photobiol. A Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A pyrene-containing Schiff base fluorescent ratiometric probe for the detection of Cu2+ in aqueous solutions and in cells
Journal of Photochemistry and Photobiology A: Chemistry ( IF 4.1 ) Pub Date : 2020-12-07 , DOI: 10.1016/j.jphotochem.2020.113086
Li-Jun Ma , Qingjian Liang , Ruihong Feng , Zu Lv , Fengchao Cui , Ligui Li , Liting Yang , Hong Liu , Fengqiang Sun

A highly selective and sensitive fluorescent ratiometric probe (1) based on pyrene-containing Schiff base for the dection of Cu2+ was synthesized. In acetonitrile–HEPES buffer solution (20.0 mM HEPES, 8:2, v/v, pH 7.4), only Cu2+, out of tested common cations, could induce the aggregation of probe 1. Fluorescence analysis showed that the excimer emission band of pyrene groups of probe 1 appeared and gradually increased with the addition of Cu2+. The fluorescent intensity ratio of excimer and monomer (I470/I409) of probe 1 increased 27.8-fold upon the addition of one equiv. of Cu2+ with a detection limit of 20.2 nM (1.28 ppb). The addition of Cu2+ also induced obvious color change and the characteristic peaks of pyrene excimer in UV–vis spectrometra of the relutant solution. Results from the Job plot, metal-binding fluorescent titration, mass spectra experiments and density functional theory calculations indicated a binding ratio of 2:1 (probe 1: Cu2+). In addition, probe 1 showed good potential as a fluorescent visualizing probe for the detection of Cu2+ in living GS cells of epinepheluscoioides.



中文翻译:

含pyr的席夫碱荧光比率探针,用于检测水溶液和细胞中的Cu 2+

合成了基于含selective席夫碱的Cu 2+的高选择性和灵敏的荧光比例探针(1)。在乙腈-HEPES缓冲溶液(20.0 mM HEPES,8:2,v / v,pH 7.4)中,只有铜2+(测试的常见阳离子中)可以诱导探针1聚集。荧光分析表明,探针1的pyr基的准分子发射带随着Cu 2+的加入而逐渐增加。添加1当量后,探针1的准分子和单体的荧光强度比(I 470 / I 409)增加了27.8倍。铜2+,检出限为20.2 nM(1.28 ppb)。Cu 2+的添加还引起稀释液的UV-vis光谱中明显的颜色变化和of准分子的特征峰。作业图,金属结合荧光滴定,质谱实验和密度泛函理论计算的结果表明结合比为2:1(探针1:Cu 2+)。另外,探针1显示出作为荧光可视化探针的良好潜力,用于检测肾上腺皮质激素的活GS细胞中的Cu 2+

更新日期:2020-12-21
down
wechat
bug