A highly selective and sensitive fluorescent ratiometric probe (1) based on pyrene-containing Schiff base for the dection of Cu²⁺ was synthesized. In acetonitrile–HEPES buffer solution (20.0 mM HEPES, 8:2, v/v, pH 7.4), only Cu²⁺, out of tested common cations, could induce the aggregation of probe 1. Fluorescence analysis showed that the excimer emission band of pyrene groups of probe 1 appeared and gradually increased with the addition of Cu²⁺. The fluorescent intensity ratio of excimer and monomer (I₄₇₀/I₄₀₉) of probe 1 increased 27.8-fold upon the addition of one equiv. of Cu²⁺ with a detection limit of 20.2 nM (1.28 ppb). The addition of Cu²⁺ also induced obvious color change and the characteristic peaks of pyrene excimer in UV–vis spectrometra of the relutant solution. Results from the Job plot, metal-binding fluorescent titration, mass spectra experiments and density functional theory calculations indicated a binding ratio of 2:1 (probe 1: Cu²⁺). In addition, probe 1 showed good potential as a fluorescent visualizing probe for the detection of Cu²⁺ in living GS cells of epinepheluscoioides.

合成了基于含selective席夫碱的Cu ²⁺的高选择性和灵敏的荧光比例探针（1）。在乙腈-HEPES缓冲溶液（20.0 mM HEPES，8：2，v / v，pH 7.4）中，只有铜²⁺（测试的常见阳离子中）可以诱导探针1聚集。荧光分析表明，探针1的pyr基的准分子发射带随着Cu ²⁺的加入而逐渐增加。添加1当量后，探针1的准分子和单体的荧光强度比（I ₄₇₀ / I ₄₀₉）增加了27.8倍。铜²⁺，检出限为20.2 nM（1.28 ppb）。Cu ^2+的添加还引起稀释液的UV-vis光谱中明显的颜色变化和of准分子的特征峰。作业图，金属结合荧光滴定，质谱实验和密度泛函理论计算的结果表明结合比为2：1（探针1：Cu ²⁺）。另外，探针1显示出作为荧光可视化探针的良好潜力，用于检测肾上腺皮质激素的活GS细胞中的Cu ²⁺。