A broiler study was conducted to compare the effectiveness of nonstarch polysaccharides-degrading enzyme (NSPase) in the presence of high dose of phytase, by comparing the effect of phytase alone or in combination, on growth performance, bone mineralization, and carcass traits. In total, 4,536 day-old male broiler birds were randomly allocated into 9 dietary treatments with 14 replicates and 36 birds in each/per replicate. A standard diet was used as a benchmark (positive control [PC]), containing corn, soybean meal, and corn distillers dried grains with solubles to meet the requirement for normal growth, followed by a 2 × 4 factorial design, namely 2 levels of nutrition (negative control [NC] 1, reduced AME 75 kcal/kg, digestible amino acids (DAA) 3%, calcium and available phosphorus [Ca and avP] 0.15 percental units; NC2, reduced AME 150 kcal/kg, DAA 6% and Ca and avP 0.20 percental units), and 4 enzyme programs: 1) no added enzymes; 2) phytase alone (1,000 FTU/kg, Buttiauxella sp.); 3) phytase (1,000 FTU/kg, Buttiauxella sp.) + NSPase (1,250 VU/kg of Talaromyces versatilis, Rovabio Advance T) as 2 separate enzyme preparations; and 4) 2 enzymes of the same origin and activity level but premerged as 1 enzyme preduct (2 in 1 Combo, Rovabio Advance Phy T), to gain logistic and cost advantages over 3). The diets were fed in 3 phases as starter (day 0–10), grower (day 11–21), and finisher (day 22–35). The results showed that birds responded proportionally to the tiered reduction of nutrients on weight gain (2,375, 2,313, and 2,222 g, P < 0.05), feed efficiency (mortality-corrected feed conversion ratio [mFCR] 1.533, 1.596, and 1.677, P < 0.05), and performance index (PI) (437, 409, and 375, P < 0.05) for PC, NC1, and NC2, respectively. The birds also responded to the enzyme program accordingly: the addition of phytase alone improved both mFCR and bone mineralization (P < 0.05), further supplementing with NSPase significantly (P < 0.05) improved overall performance including growth rate, mFCR, and PI. Interactions were observed between the levels of nutrients and enzyme programs. Supplementing both phytase and NSPase showed more strength to help birds to recover mFCR than using phytase alone. For NC1, while using phytase alone recovered 8 PI points, inclusion of both phytase and NSPase restored PI to the PC level; For NC2, despite the addition of both enzymes recovered 20 PI points (P < 0.05), none of enzyme programs was able to restore PI to the PC level. The results suggest the phytase and NSPase function synergistically in the gastrointestinal tract, resulting in improved digestion and absorption of nutrients.

肉鸡研究进行了比较，通过比较植酸酶单独或组合对生长性能，骨矿化和car体性状的影响，比较了在高剂量植酸酶存在下非淀粉多糖降解酶（NSPase）的有效性。总共将4,536日龄的雄性肉仔鸡随机分配到9种饮食处理中，每组14个重复，每重复36个鸡。以标准饮食为基准（阳性对照[ PC ]），其中包含玉米，豆粕和玉米蒸馏器可溶谷物，以符合正常生长的需要，然后是2×4析因设计，即2级营养（阴性对照[ NC ] 1，降低的AME 75 kcal / kg，可消化的氨基酸（DAA）3％，钙和有效磷[ Ca和avP ] 0.15个百分点单位；NC2，降低的AME 150 kcal / kg，DAA 6％和Ca和avP 0.20百分比单位）和4种酶程序：1）不添加酶；2）单独的植酸酶（1,000 FTU / kg，Buttiauxella sp。）；3）肌醇六磷酸酶（FTU 1000 /公斤，布丘氏菌属物种）+ NSPase（1250 VU /公斤的踝节菌属versatilis，Rovabio Advance T）作为2种独立的酶制剂；和4）2种具有相同起源和活性水平的酶，但预先作为1种酶的前体（2合1组合，Rovabio Advance Phy T），比3种在物流和成本方面更具优势。日粮分三个阶段进行饲喂，即开始（0-10天），生长（11-21天）和完成（22-35天）。结果表明，家禽对体重增加（2,375、2,313和2,222 g，P <0.05），饲料效率（按死亡率校正的饲料转化率[ mFCR ] 1.533、1.596和1.677，P <0.05）和性能指标（PI）（437、409和375，P<0.05），分别适用于PC，NC1和NC2。鸟类也对酶程序做出了相应的反应：单独添加植酸酶可以改善mFCR和骨矿化（P <0.05），进一步补充NSPase（P <0.05）可以改善包括生长速率，mFCR和PI在内的总体性能。营养水平和酶程序之间存在相互作用。与单独使用肌醇六磷酸酶相比，同时添加肌醇六磷酸酶和NSPase可以显示更多的强度来帮助鸟类恢复mFCR。对于NC1，仅使用肌醇六磷酸酶可恢复8个PI点，同时包含肌醇六磷酸酶和NSPase可将PI恢复至PC水平。对于NC2，尽管同时添加了两种酶，但仍可恢复20个PI点（P<0.05），没有酶程序能够将PI恢复到PC水平。结果表明，植酸酶和NSPase在胃肠道中具有协同作用，从而改善了营养物质的消化吸收。