ABSTRACT

Y-box binding proteins are members of the family of proteins containing the evolutionarily conserved cold shock domain. Their cellular functions are quite diverse, including transcription and translation regulation, participation in pre-mRNA splicing, mRNA stabilization and packaging into mRNPs, involvement in DNA repair, and some others. To date, we know little about the plausible functional interchangeability of Y-box binding proteins. Our previous finding was that in YB-1-null HEK293T cells the synthesis of YB-3 is enhanced, thus enabling YB-3 to interact with a larger set of mRNAs and compensate for the YB-1 absence. We suggested the existence of a mechanism of YB-3 synthesis regulation by its paralog, YB-1. Here we demonstrate that YB-1 participates in the translational control and stabilization of YB-3 mRNA through untranslated regions of YB-3 mRNA.

摘要

Y-box 结合蛋白是包含进化上保守的冷休克结构域的蛋白质家族的成员。它们的细胞功能非常多样化，包括转录和翻译调节、参与前 mRNA 剪接、mRNA 稳定和包装成 mRNP、参与 DNA 修复等。迄今为止，我们对 Y-box 结合蛋白的合理功能互换性知之甚少。我们之前的发现是，在 YB-1-null HEK293T 细胞中，YB-3 的合成增强，从而使 YB-3 能够与更大的 mRNA 相互作用并补偿 YB-1 的缺失。我们建议存在通过其旁系同源物 YB-1 调节 YB-3 合成的机制。在这里，我们证明 YB-1 参与了YB-3的平移控制和稳定mRNA 通过YB-3 mRNA 的非翻译区。