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Two atypical ANGUSTIFOLIA without a plant‐specific C‐terminus regulate gametophore and sporophyte shapes in the moss Physcomitrium (Physcomitrella) patens
The Plant Journal ( IF 6.2 ) Pub Date : 2020-12-06 , DOI: 10.1111/tpj.15121 Katsuaki Takechi 1 , Hiroaki Nagase 2 , Tomoyuki Furuya 3, 4 , Koro Hattori 3 , Yoshikatsu Sato 5 , Kensuke Miyajima 2 , Tomofumi Higuchi 2 , Ryuya Matsuda 6 , Susumu Takio 1, 6 , Hirokazu Tsukaya 3 , Hiroyoshi Takano 1
The Plant Journal ( IF 6.2 ) Pub Date : 2020-12-06 , DOI: 10.1111/tpj.15121 Katsuaki Takechi 1 , Hiroaki Nagase 2 , Tomoyuki Furuya 3, 4 , Koro Hattori 3 , Yoshikatsu Sato 5 , Kensuke Miyajima 2 , Tomofumi Higuchi 2 , Ryuya Matsuda 6 , Susumu Takio 1, 6 , Hirokazu Tsukaya 3 , Hiroyoshi Takano 1
Affiliation
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ANGUSTIFOLIA (AN) is a plant‐specific subfamily of the CtBP/BARS/AN family, characterized by a plant‐specific C‐terminal domain of approximately 200 amino acids. Previously, we revealed that double knockout (DKO) lines of Physcomitrium (Physcomitrella) patens ANGUSTIFOLIA genes (PpAN1‐1 and PpAN1‐2) show defects in gametophore height and the lengths of the seta and foot region of sporophytes, by reduced cell elongation. In addition to two canonical ANs, the genome of P. patens has two atypical ANs without a coding region for a plant‐specific C‐terminus (PpAN2‐1 and PpAN2‐2); these were investigated in this study. Similar to PpAN1s, both promoters of the PpAN2 genes were highly active in the stems of haploid gametophores and in the middle‐to‐basal region of young diploid sporophytes that develop into the seta and foot. Analyses of PpAN2‐1/2‐2 DKO and PpAN quadruple knockout (QKO) lines implied that these four AN genes have partially redundant functions to regulate cell elongation in their expression regions. Transgenic strains harboring P. patens α‐tubulin fused to green fluorescent protein, which were generated from a QKO line, showed that the orientation of the microtubules in the gametophore tips in the PpAN QKO lines was unchanged from the wild‐type and PpAN1‐1/1‐2 DKO plants. In addition to both PpAN2‐1 and PpAN2‐2, short Arabidopsis AN without the C‐terminus of 200 amino acids could rescue the Arabidopsis thaliana an‐1 phenotypes, implying AN activity is dependent on the N‐terminal regions.
中文翻译：
两种不具有植物特异性C末端的非典型AngUSTIFOLIA调节苔藓小体（Physcomitrella）掌中的配子体和孢子体形状
ANGUSTIFOLIA（AN）是CtBP / BARS / AN家族的植物特异性亚科，其特征是具有约200个氨基酸的植物特异性C末端结构域。以前，我们揭示了通过减少细胞伸长，Physcomitrium（Physcomitrella）patens ANGUSTIFOLIA基因（PpAN1-1和PpAN1-2）的双敲除（DKO）品系显示了配子体高度以及子孢子的刚毛和足部区域长度的缺陷。除了两个典型的AN，P的基因组。patens具有两个非典型的AN，没有针对植物的C端（PpAN2-1和PpAN2-1）的编码区。PpAN2-2）; 这些在本研究中进行了调查。与PpAN1相似，PpAN2基因的两个启动子在单倍体配体的茎中以及在发育成刚毛和足部的幼小二倍体孢子体的中-基础区域中都具有很高的活性。对PpAN2-1 / 2 / 2-2 DKO和PpAN四重敲除（QKO）品系的分析表明，这四个AN基因在调节其表达区的细胞伸长方面具有部分冗余功能。携带P的转基因菌株。帕滕斯从QKO系产生的与绿色荧光蛋白融合的α-微管蛋白表明，PpAN QKO系配子体尖端中微管的方向与野生型和PpAN1-1 / 1-2 DKO植物相同。除了PpAN2-1和PpAN2-2以外，没有200个氨基酸C末端的短拟南芥AN可以拯救拟南芥an-1表型，这意味着AN活性取决于N末端区域。
更新日期：2020-12-06
中文翻译：
两种不具有植物特异性C末端的非典型AngUSTIFOLIA调节苔藓小体（Physcomitrella）掌中的配子体和孢子体形状
ANGUSTIFOLIA（AN）是CtBP / BARS / AN家族的植物特异性亚科，其特征是具有约200个氨基酸的植物特异性C末端结构域。以前，我们揭示了通过减少细胞伸长，Physcomitrium（Physcomitrella）patens ANGUSTIFOLIA基因（PpAN1-1和PpAN1-2）的双敲除（DKO）品系显示了配子体高度以及子孢子的刚毛和足部区域长度的缺陷。除了两个典型的AN，P的基因组。patens具有两个非典型的AN，没有针对植物的C端（PpAN2-1和PpAN2-1）的编码区。PpAN2-2）; 这些在本研究中进行了调查。与PpAN1相似，PpAN2基因的两个启动子在单倍体配体的茎中以及在发育成刚毛和足部的幼小二倍体孢子体的中-基础区域中都具有很高的活性。对PpAN2-1 / 2 / 2-2 DKO和PpAN四重敲除（QKO）品系的分析表明，这四个AN基因在调节其表达区的细胞伸长方面具有部分冗余功能。携带P的转基因菌株。帕滕斯从QKO系产生的与绿色荧光蛋白融合的α-微管蛋白表明，PpAN QKO系配子体尖端中微管的方向与野生型和PpAN1-1 / 1-2 DKO植物相同。除了PpAN2-1和PpAN2-2以外，没有200个氨基酸C末端的短拟南芥AN可以拯救拟南芥an-1表型，这意味着AN活性取决于N末端区域。
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