Salinity and drought conditions commonly result in osmotic and oxidative stresses, while salinity additionally causes ionic stress. In this study, we identified specific genes regulated by osmotic and ionic stresses in five Arabidopsis ecotypes. SHA and C24 ecotypes were more tolerant to salt and drought stresses at the seedling growth stage, as evidenced by lower water loss rate, lower electrolyte leakage and higher survival rate when compared to the other three ecotypes under drought and salinity conditions. Transcriptomic analysis revealed that 3700 and 2242 genes were differentially regulated by salt and osmotic stresses, respectively. Totally 78.1% of upregulated and 62.0% of downregulated genes by osmotic stress were also commonly regulated by salt stress. GO term enrichment analysis showed that auxin (IAA), abscisic acid, cytokinin and gibberellic acid pathways were regulated by the osmotic stress, while IAA, jasmonic acid and ethylene pathways were changed by the ionic stress. The nutrient and water uptake pathways were regulated by both the osmotic and ionic stresses, whereas ion transportation and kinase pathways were modulated by the ionic stress. Additionally, we characterized bHLH61 as a negative regulator in response to salt and drought stresses. This study provided new clues of plant responses to salt and drought stresses. This article is protected by copyright. All rights reserved.

中文翻译：

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拟南芥生态型响应干旱和盐胁迫的转录变异分析剖析了常见的调控网络

盐度和干旱条件通常会导致渗透和氧化应激，而盐度还会导致离子应激。在这项研究中，我们在五个拟南芥生态型中鉴定了受渗透和离子胁迫调节的特定基因。SHA 和 C24 生态型在幼苗生长阶段对盐和干旱胁迫的耐受性更高，与干旱和盐度条件下的其他三种生态型相比，其失水率更低、电解质泄漏更低和存活率更高。转录组学分析显示，3700 和 2242 个基因分别受到盐和渗透胁迫的差异调节。总共有 78.1% 的渗透胁迫上调基因和 62.0% 下调基因通常也受盐胁迫的调控。GO 术语富集分析表明，生长素（IAA）、脱落酸、细胞分裂素和赤霉酸途径受渗透胁迫调节，而IAA、茉莉酸和乙烯途径受离子胁迫改变。营养和水分摄取途径受渗透和离子胁迫调节，而离子转运和激酶途径受离子胁迫调节。此外，我们将 bHLH61 表征为响应盐和干旱胁迫的负调节因子。这项研究为植物对盐和干旱胁迫的反应提供了新的线索。本文受版权保护。版权所有。而离子转运和激酶途径受离子应力调节。此外，我们将 bHLH61 表征为响应盐和干旱胁迫的负调节因子。这项研究为植物对盐和干旱胁迫的反应提供了新的线索。本文受版权保护。版权所有。而离子转运和激酶途径受离子应力调节。此外，我们将 bHLH61 表征为响应盐和干旱胁迫的负调节因子。这项研究为植物对盐和干旱胁迫的反应提供了新的线索。本文受版权保护。版权所有。