Pardosa pseudoannulata is the main predatory natural enemy of crop pests in a paddy ecosystem. When P. pseudoannulata is exposed to unfavorable temperature conditions, the response of heat shock proteins could resist the damage, and is therefore, conducive to the organism’s rapid adaptation to the surrounding stress environment. In this study, we explored the roles of hsp70 and hsp90 genes in response to heat stress, using the rapid amplification of cDNA ends technique and cloned full-length cDNAs of Pphsp70, Pphsp83, and Pphsp90. The mRNA expression levels of the three genes under different temperature stresses (25, 28, 31, 34, 37, 40, and 43 °C) and with different duration stresses (4, 8, 12, 16, and 20 h) were analyzed by quantitative real-time polymerase chain reaction. The full-length cDNA of Pphsp70, Pphsp83, and Pphsp90 was 2331 base pair (bp), 2466 bp, and 2663 bp, respectively. Phylogenetic analysis of amino acid sequences of Pphsp70, Pphsp83, and Pphsp90 showed that the sequences had high homology with that of other spiders. The mRNA expression of all three genes was extremely significantly up-regulated at 43 °C. Moreover at 43 °C, the expression of all three genes in both female and male spiders at the duration of 4 h was the highest compared to that of other stress duration groups. Therefore, it can be inferred that the three genes of P. pseudoannulata play a crucial protective role in resistance in a high-temperature environment.

稻纵卷叶is是稻田生态系统中农作物害虫的主要掠食性天敌。当P。假单胞菌暴露于不利的温度条件下，热激蛋白的反应可以抵抗损伤，因此有利于生物体快速适应周围的胁迫环境。在这项研究中，我们使用cDNA末端快速扩增技术并克隆了Pphsp70，Pphsp83和Pphsp90的全长cDNA，探索了hsp70和hsp90基因在热应激中的作用。。分析了三个基因在不同温度胁迫（25、28、31、34、37、40和43°C）和不同持续时间胁迫（4、8、12、16和20 h）下的mRNA表达水平通过定量实时聚合酶链反应。Pphsp70，Pphsp83和Pphsp90的全长cDNA分别为2331个碱基对（bp），2466 bp和2663 bp。系统发育分析的氨基酸序列的Pphsp70，Pphsp83和Pphsp90结果表明该序列与其他蜘蛛具有高度同源性。在43°C时，所有三个基因的mRNA表达均显着上调。此外，在43°C时，与其他胁迫持续时间组相比，雌性和雄性蜘蛛在4 h时所有三个基因的表达最高。因此，可以推断出假单胞菌的三个基因在高温环境下在抗性中起着至关重要的保护作用。