During the infection and colonization process, the rice blast fungus Magnaporthe oryzae faces various challenges from hostile environment, such as nutrient limitation and carbon stress, while carbon catabolite repression (CCR) mechanism would facilitate the fungus to shrewdly and efficiently utilize carbon nutrients under fickle nutritional conditions since it ensures the preferential utilization of most preferred carbon sources through repressing the expression of enzymes required for the utilization of less preferred carbon sources. Researches on M. oryzae CCR have made some progress, however the involved regulation mechanism is still largely obscured, especially, little is known about the key carbon catabolite repressor CreA. Here we identified and characterized the biological functions of the CreA homolog MoCreA in M. oryzae. MoCreA is constitutively expressed throughout all the life stages of the fungus, and it can shuttle between nucleus and cytoplasm which is induced by glucose. Following functional analyses of MoCreA suggested that it was required for the vegetative growth, conidiation, appressorium formation and pathogenicity of M. oryzae. Moreover, comparative transcriptomic analysis revealed that disruption of MoCreA resulted in the extensive gene expression variations, including a large number of carbon metabolism enzymes, transcription factors and pathogenicity-related genes. Taken together, our results demonstrated that, as a key regulator of CCR, MoCreA plays a vital role in precise regulation of the asexual development and pathogenicity of the rice blast fungus.

在感染和定植过程中，稻瘟病菌Magnaporthe oryzae面临来自恶劣环境的各种挑战，如营养限制和碳胁迫，而碳分解代谢物抑制 (CCR) 机制将促进真菌在营养变化无常的情况下精明有效地利用碳营养。条件，因为它通过抑制利用较不优选的碳源所需的酶的表达来确保优先利用最优选的碳源。米曲霉的研究CCR 取得了一些进展，但所涉及的调控机制仍然在很大程度上模糊不清，尤其是对关键的碳分解代谢物阻遏物 CreA 知之甚少。在这里，我们鉴定并表征了米曲霉中CreA 同源物 MoCreA 的生物学功能。MoCreA 在真菌的所有生命阶段都呈组成型表达，它可以在葡萄糖诱导的细胞核和细胞质之间穿梭。MoCreA 的功能分析表明它是米曲霉的营养生长、分生孢子、附着物形成和致病性所必需的。此外，比较转录组学分析表明，MoCreA 的破坏导致广泛的基因表达变异，包括大量的碳代谢酶、转录因子和致病相关基因。总之，我们的结果表明，作为 CCR 的关键调节剂，MoCreA 在精确调控稻瘟病真菌的无性发育和致病性方面起着至关重要的作用。