Sargassum horneri is a popular, and edible seaweed known for its biofunctional properties. Extracts of S. horneri were recently studied owing to the therapeutic potential in the remediation of inflammatory diseases. The present study evaluated the inhibitory effects of S. horneri 70% ethanol extracts (SHE) against tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced inflammation in HaCaT keratinocytes and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. SHE reduced the levels of epidermal and epithelial innate cytokines, interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin and inflammatory cytokines, IL-1β, IL-4, IL-6, IL-13, IFN-γ, and TNF-α in TNF-α/IFN-γ-stimulated HaCaT keratinocytes. Also, SHE decreased the expression levels of inflammatory chemokines, Eotaxin, a macrophage-derived chemokine, expressed and secreted normal T-cells, and thymus and activation regulated chemokine. Moreover, SHE suppressed the activation of nuclear factor-κB p65 signaling in HaCaT keratinocytes as well as the activation of extracellular signal-regulated kinase/p38 signaling. SHE enhanced the nuclear factor erythroid-2-related factor/heme oxygenase-mediated antioxidant defense system. SHE also reduced the ear thickness, infiltration of inflammatory cells, hyperplasia, and hyperkeratosis in the TPA-induced ear edema mouse model. The anti-inflammatory effects of SHE were implicated in the anti-inflammatory effects of SHE in TNF-α/IFN-γ-induced inflammation in HaCaT keratinocytes and TPA-induced ear edema in mice. This study suggested that SHE has anti-inflammatory effects on TNF-α/IFN-γ-induced inflammation in keratinocytes and TPA-induced ear edema in a mice model. Further research could develop SHE as a food supplement/nutricosmetic to enhance protective effects against inflammatory skin diseases.

马尾藻（Sargassum horneri）是一种流行的可食用海藻，以其生物功能特性而闻名。由于在治疗炎性疾病方面具有治疗潜力，最近研究了S. horneri提取物。本研究评估了霍恩沙门氏菌70％乙醇提取物（SHE）对肿瘤坏死因子（TNF）-α/干扰素（IFN）-γ诱导的HaCaT角质形成细胞和12- O炎症的抑制作用-十四烷酰phorbol-13-乙酸酯（TPA）引起的小鼠耳部水肿。SHE降低了表皮和上皮先天细胞因子，白介素（IL）-25，IL-33和胸腺基质淋巴细胞生成素和炎性细胞因子，IL-1β，IL-4，IL-6，IL-13，IFN-γ， TNF-α/IFN-γ刺激的HaCaT角质形成细胞中的TNF-α和TNF-α。此外，SHE降低了炎症趋化因子，趋化因子，巨噬细胞衍生的趋化因子，正常T细胞的表达和分泌以及胸腺和激活调节趋化因子的表达水平。此外，SHE抑制了HaCaT角质形成细胞中核因子κBp65信号的激活以及细胞外信号调节激酶/ p38信号的激活。SHE增强了核因子红系2相关因子/血红素加氧酶介导的抗氧化防御系统。SHE还减少了耳朵的厚度，TPA诱发的耳部水肿小鼠模型中炎症细胞的浸润，增生和过度角化。SHE的抗炎作用与SHE对TNF-α/IFN-γ诱导的HaCaT角质形成细胞的炎症和TPA诱导的小鼠耳水肿的抗炎作用有关。这项研究表明，SHE对小鼠模型中的TNF-α/IFN-γ诱导的角质形成细胞炎症和TPA诱导的耳水肿具有抗炎作用。进一步的研究可以开发SHE作为食品补充剂/营养保健品，以增强针对炎症性皮肤病的保护作用。SHE的抗炎作用与SHE对TNF-α/IFN-γ诱导的HaCaT角质形成细胞的炎症和TPA诱导的小鼠耳水肿的抗炎作用有关。这项研究表明，SHE对小鼠模型中的TNF-α/IFN-γ诱导的角质形成细胞炎症和TPA诱导的耳水肿具有抗炎作用。进一步的研究可以开发SHE作为食品补充剂/营养保健品，以增强针对炎症性皮肤病的保护作用。SHE的抗炎作用与SHE对TNF-α/IFN-γ诱导的HaCaT角质形成细胞的炎症和TPA诱导的小鼠耳水肿的抗炎作用有关。这项研究表明，SHE对小鼠模型中的TNF-α/IFN-γ诱导的角质形成细胞炎症和TPA诱导的耳水肿具有抗炎作用。进一步的研究可以开发SHE作为食品补充剂/营养保健品，以增强针对炎症性皮肤病的保护作用。