The present investigation was targeted towards a highly desirable everbearing trait in strawberry (Fragaria × ananassa Duch.) via marker assisted selection while seeing its worldwide commercial applicability through the extended harvest season. The crosses were made between everbearing and june-bearing cultivars to raise the F₁ individuals. Morphological characters (plant, floral, and fruit) were assessed that showed significant differences among the strawberry cultivars. Molecular characterization was carried out between everbearing and non-everbearing cultivars using RAPD and SSR markers. For phenotyping, a chi-square test was performed and revealed that out of all four cross combinations, the best fitted cross found to be in Mendelian segregation ratio (1:1) was ‘Confectura’ × ‘Torrey’ with χ²-value 1.58. Further, the identified polymorphic markers were assessed across the F₁ individuals of cross ‘Confectura’ × ‘Torrey’ for its genotyping. It could be revealed that the targeted everbearing trait is governed by a dominant gene(s) in the subjected strawberry genotypes. Further, the identified polymorphic markers would be successfully employed in DNA fingerprinting of strawberry under various crop improvement programme.

本研究通过标记辅助选择针对草莓 ( Fragaria  ×  ananassa Duch.) 中非常理想的永生性状，同时通过延长的收获季节看到其在全球范围内的商业适用性。在常年和6月品种之间进行杂交以提高F ₁个人。对形态特征（植物、花和果实）进行了评估，发现草莓品种之间存在显着差异。使用 RAPD 和 SSR 标记在常年和非常年品种之间进行分子表征。对于表型分析，进行了卡方检验，结果显示在所有四种杂交组合中，发现孟德尔分离比 (1:1) 的最佳拟合杂交是 'Confectura' × 'Torrey'， χ ²值为1.58 . _{此外，在 F 1}中评估了鉴定的多态性标记杂交 'Confectura' × 'Torrey' 的个体进行基因分型。可以揭示，目标永生性状由受试草莓基因型中的显性基因控制。此外，在各种作物改良计划下，鉴定出的多态性标记将成功地用于草莓的 DNA 指纹识别。