Populus spp. have long been used as model woody plant species for molecular biology research. However, tissues of poplar are often recalcitrant to experimental procedures for molecular studies. We generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba × P. berolinensis ‘Yinzhong’, named Qu-2. Qu-2 cells are suitable as a model biological system for studying woody plants. Qu-2 cells have many advantages over suspension cell lines derived so far from any other woody plants. Qu-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant hormone. They show exceptionally high growth rates, reaching an approximately 150-fold increase in biomass after one week of culturing. Another important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily reactivated. Qu-2 cells are suitable for molecular manipulations such as protoplast production, transient transformation, and RNA-seq analysis. Therefore, Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research, ranging from physiology to gene function. The Qu-2 cells will be made available to the plant community for research.

杨属。长期以来一直用作分子生物学研究的模型木本植物物种。然而，杨树的组织经常对分子研究的实验程序不利。我们从白杨×柏林杨的杂种产生了一种激素自养杨树悬浮细胞系“银钟”，名为Qu-2。Qu-2细胞适合作为研究木本植物的模型生物学系统。Qu-2细胞比迄今从未有其他木本植物衍生的悬浮细胞系具有许多优势。Qu-2细胞非常易于培养，可以在几种常见的植物培养基上生长，而无需添加任何植物激素。它们显示出极高的生长速度，培养一周后生物量增加了约150倍。Qu-2细胞的另一个重要的独特特征是它们可以冷冻保存并易于重新激活。Qu-2细胞适用于分子操作，例如原生质体生产，瞬时转化和RNA-seq分析。因此，Qu-2细胞具有成为树分子生物学研究中优良模型细胞的巨大潜力，从生理学到基因功能。Qu-2细胞将提供给植物界进行研究。