The hybrids of pimelodid catfish provide products of excellent genetic value for aquaculture due to their greater growth in size, resistance to diseases and adaptation to feed concentrate. However, this practice involves risks such as genetic contamination, the commercialization of hybrid products as pure species, the introduction of exotic species and escapes of fish farming products. Thus, the aim of this study was to evaluate microsatellites for the identification of hybrids between Pseudoplatystoma metaense and Leiarius marmoratus . Genomic DNA extraction was achieved from fin tissue and nine heterologous microsatellites previously reported in the literature were amplified: Pcor01, Pcor02, Pcor05, Pcor10, Phrac02, Phrac07, Phrac09, Prt03, and Prt30. The DNA extraction resulted in high concentrations and a good DNA quality with absorbance values at 260/280 nm between 1.96 and 2.07. The microsatellites Pcor10, Phrac02, Phrac07 and Prt03 indicated heterozygosity patterns in the agarose gel for hybrids, with the presence of two diagnostic bands. Our results showed that these microsatellites are a powerful and economic tool for identifying interspecific hybridization, without requiring genotyping or sequencing. Here, we made the first report of amplification of these microsatellites for the endemic species of the Orinoco basin Pseudoplatystoma metaense , useful information for population and phylogeny studies. We also highlight the importance of using molecular identification techniques for hybrids and we invite the aquaculture industry to conserve good management practices in the fish farming areas. Thereby, hybrids do not compromise the sustainable development of aquaculture or pose serious risks for native and wild species.

中文翻译：

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一种利用微卫星技术鉴定 cat鱼杂种的经济方法：以 Pseudoplatystoma metaense × Leiarius marmoratus 杂种为例

吡美洛德hybrid鱼的杂种由于其尺寸长大，对疾病的抵抗力和对饲料浓缩物的适应性，为水产养殖提供了具有优良遗传价值的产品。但是，这种做法涉及诸如遗传污染，将杂种产品作为纯种商业化，引入外来物种以及鱼类养殖产品逃逸之类的风险。因此，本研究的目的是评估微卫星，以鉴定间 质假单胞菌和 沙氏Learius marmoratus 之间的杂种。 。从鳍组织中提取基因组DNA，并扩增了先前文献中报道的九种异源微卫星：Pcor01，Pcor02，Pcor05，Pcor10，Phrac02，Phrac07，Phrac09，Prt03和Prt30。DNA提取产生高浓度和良好的DNA质量，在260/280 nm处的吸光度值为1.96至2.07。微卫星Pcor10，Phrac02，Phrac07和Prt03表示杂种在琼脂糖凝胶中的杂合性模式，存在两个诊断带。我们的结果表明，这些微卫星是鉴定种间杂交的强大而经济的工具，而无需进行基因分型或测序。在这里，我们首次报道了这些微卫星对奥里诺科盆地特有物种的扩增 Pseudoplatystoma metaense ，对于种群和系统发育研究有用的信息。我们还强调了对杂交种使用分子识别技术的重要性，并邀请水产养殖业在养鱼区保存良好的管理规范。因此，杂种不会损害水产养殖业的可持续发展，也不会对本地和野生物种构成严重威胁。