The brown planthopper (BPH), Nilaparvata lugens Stål, is one of the most destructive pests to the rice production in the world. Thus, there is an urgency to identify new resistant genes for breeding. AC-1613 is an indica variety that has been reported to confer broad-spectrum resistance to BPH. In the present study, we found that AC-1613 exhibited strong antibiosis towards BPH insects. The body weight was significantly decreased when the insects fed on AC-1613 plants. By using BPH weight gain as an index of phenotyping, a novel dominant locus for resistance to BPH, designed as Bph30 , was identified and its near-isogenic line (NIL) in 9311 background was developed. The F₂ population derived from a cross between AC-1613 and 9311 was used for mapping the gene. Through QTL scan, we located the gene on the short arm of chromosome 4 between RM16278 and RM16425, which explained 42.7% of the phenotypic variance (PEV) of BPH resistance in the F₂ population. The gene was finally located in a region flanking by simple sequence repeat (SSR) markers SSR-28 and SSR-69 through high-resolution mapping, the distance between the two markers in Nipponbare genome is 37.5 kb. In addition, SSR markers RM16294 and RM16299 tightly linked to Bph30 were applied effectively in introgressing Bph30 into elite rice cultivars. The developed NILs showed a strong antibiosis and high resistance to BPH.

中文翻译：

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对9311背景赋予棕色飞虱强烈抗生并发展抗近等基因系的基因的高分辨率定位

褐飞虱（ Nilaparvata lugensStål）是世界水稻生产中最具破坏力的害虫之一。因此，迫切需要鉴定新的抗性基因进行育种。AC-1613是一种 in稻 ，据报道可赋予BPH广谱抗性。在本研究中，我们发现AC-1613对BPH昆虫表现出强烈的抗菌作用。当昆虫以AC-1613植物为食时，体重显着降低。以BPH增重为表型指标，鉴定了一种新的抗BPH优势基因座， 命名为 Bph30 ，并在9311背景下开发了其近等基因系（NIL）。F ₂从AC-1613和9311之间的杂交获得的群体被用于该基因的定位。通过QTL扫描，我们将该基因定位在4号染色体的短臂上，介于RM16278和RM16425之间，这解释了F ₂人群BPH抗性的表型变异（PEV）的42.7％。该基因最终通过高分辨率定位位于简单序列重复（SSR）标记SSR-28和SSR-69侧翼的区域，日本晴基因组中两个标记之间的距离为37.5 kb。此外，与Bph30紧密连接的SSR标记RM16294和RM16299可有效地用于将 Bph30 渗入 优良 水稻品种。已开发的NIL显示出强大的抗菌性和对BPH的高抗性。